Long Non-coding RNA SNHG6 Promotes the Growth and Invasion of Non-small Cell Lung Cancer by Downregulating MiR-101-3p

Overview

Journal Thorac Cancer

Specialties Oncology
Pulmonary Medicine

Date 2020 Mar 10

PMID 32147945

Citations 15

Authors

Ke Li

Yongxin Jiang

Xudong Xiang

Quan Gong

Chunyan Zhou

Lijuan Zhang

Qianli Ma

Li Zhuang

Affiliations

Soon will be listed here.

Abstract

Background: The aim of this study was to determine the function of long non-coding RNA small nucleolar RNA host gene 6 (SNHG6) in non-small cell lung cancer (NSCLC) and its underlying mechanisms.

Methods: The association of SNHG6 or miR-101-3p with clinicopathological characteristics and prognosis in patents with NSCLC was assessed by TCGA dataset. Cell proliferation and invasion were evaluated by MTT and Transwell assays and SNHG6-specific binding with miR-101-3p was verified by bioinformatic analysis, luciferase gene report and RNA immunoprecipitation assays. qRT-PCR and Western blot was used to assess the effects of SNHG6 on the expression of miR-101-3p and chromodomain Y like (CDYL) in NSCLC cells. A xenograft tumor model in vivo was established to observe the effects of SNHG6 knockdown on tumor growth.

Results: We found that increased expression of SNHG6 was associated with pathological stage and lymph node infiltration, and acted as an independent prognostic factor of tumor recurrence in patients with NSCLC. Silencing SNHG6 expression repressed cell growth and invasion in vitro and in vivo, but overexpression of SNHG6 reversed these effects. Furthermore, SNHG6 was identified to act as a sponge of miR-101-3p, which could reduce cell proliferation and attenuate SNHG6-induced CDYL expression. Low expression of miR-101-3p or high expression of CDYL was related to poor survival in patients with NSCLC.

Conclusions: Our findings demonstrated that lncRNA SNHG6 contributed to the proliferation and invasion of NSCLC by downregulating miR-101-3p.

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References

Zhang X, He X, Liu Y, Zhang H, Chen H, Guo S . MiR-101-3p inhibits the growth and metastasis of non-small cell lung cancer through blocking PI3K/AKT signal pathway by targeting MALAT-1. Biomed Pharmacother. 2017; 93:1065-1073. DOI: 10.1016/j.biopha.2017.07.005. View

Liang R, Xiao G, Wang M, Li X, Li Y, Hui Z . SNHG6 functions as a competing endogenous RNA to regulate E2F7 expression by sponging miR-26a-5p in lung adenocarcinoma. Biomed Pharmacother. 2018; 107:1434-1446. DOI: 10.1016/j.biopha.2018.08.099. View

Ruan J, Zheng L, Hu N, Guan G, Chen J, Zhou X . Long noncoding RNA SNHG6 promotes osteosarcoma cell proliferation through regulating p21 and KLF2. Arch Biochem Biophys. 2018; 646:128-136. DOI: 10.1016/j.abb.2018.03.036. View

Zhang Y, Li R, Ding X, Zhang K, Qin W . Upregulation of long non-coding RNA SNHG6 promote esophageal squamous cell carcinoma cell malignancy and its diagnostic value. Am J Transl Res. 2019; 11(2):1084-1091. PMC: 6413246. View

Wu D, Hsu N, Wang Y, Lee M, Cheng Y, Chen C . c-Myc suppresses microRNA-29b to promote tumor aggressiveness and poor outcomes in non-small cell lung cancer by targeting FHIT. Oncogene. 2014; 34(16):2072-82. DOI: 10.1038/onc.2014.152. View

Qiu Z, Zhu W, Meng H, Tong L, Li X, Luo P . CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter. Theranostics. 2019; 9(16):4717-4729. PMC: 6643436. DOI: 10.7150/thno.33680. View

Huang Y, Zhang J, Hou L, Wang G, Liu H, Zhang R . LncRNA AK023391 promotes tumorigenesis and invasion of gastric cancer through activation of the PI3K/Akt signaling pathway. J Exp Clin Cancer Res. 2017; 36(1):194. PMC: 5745957. DOI: 10.1186/s13046-017-0666-2. View

Li L, Shao M, Zou S, Xiao Z, Chen Z . MiR-101-3p inhibits EMT to attenuate proliferation and metastasis in glioblastoma by targeting TRIM44. J Neurooncol. 2018; 141(1):19-30. DOI: 10.1007/s11060-018-2973-7. View

Liang H, Yu T, Han Y, Jiang H, Wang C, You T . LncRNA PTAR promotes EMT and invasion-metastasis in serous ovarian cancer by competitively binding miR-101-3p to regulate ZEB1 expression. Mol Cancer. 2018; 17(1):119. PMC: 6087007. DOI: 10.1186/s12943-018-0870-5. View

10.

Cao S, Lin L, Xia X, Wu H . lncRNA SPRY4-IT1 Regulates Cell Proliferation and Migration by Sponging miR-101-3p and Regulating AMPK Expression in Gastric Cancer. Mol Ther Nucleic Acids. 2019; 17:455-464. PMC: 6646863. DOI: 10.1016/j.omtn.2019.04.030. View

11.

Meng S, Jian Z, Yan X, Li J, Zhang R . LncRNA SNHG6 inhibits cell proliferation and metastasis by targeting ETS1 via the PI3K/AKT/mTOR pathway in colorectal cancer. Mol Med Rep. 2019; 20(3):2541-2548. PMC: 6691244. DOI: 10.3892/mmr.2019.10510. View

12.

Jafari-Oliayi A, Asadi M . SNHG6 is upregulated in primary breast cancers and promotes cell cycle progression in breast cancer-derived cell lines. Cell Oncol (Dordr). 2019; 42(2):211-221. DOI: 10.1007/s13402-019-00422-6. View

13.

Yu C, Sun J, Leng X, Yang J . Long noncoding RNA SNHG6 functions as a competing endogenous RNA by sponging miR-181a-5p to regulate E2F5 expression in colorectal cancer. Cancer Manag Res. 2019; 11:611-624. PMC: 6331078. DOI: 10.2147/CMAR.S182719. View

14.

Li Y, Li D, Zhao M, Huang S, Zhang Q, Lin H . Long noncoding RNA SNHG6 regulates p21 expression via activation of the JNK pathway and regulation of EZH2 in gastric cancer cells. Life Sci. 2018; 208:295-304. DOI: 10.1016/j.lfs.2018.07.032. View

15.

Li B, Xie D, Zhang H . MicroRNA-101-3p advances cisplatin sensitivity in bladder urothelial carcinoma through targeted silencing EZH2. J Cancer. 2019; 10(12):2628-2634. PMC: 6584933. DOI: 10.7150/jca.33117. View

16.

Siegel R, Miller K, Jemal A . Cancer statistics, 2018. CA Cancer J Clin. 2018; 68(1):7-30. DOI: 10.3322/caac.21442. View

17.

Shi S, Zhang Z . Long non-coding RNA SNHG1 contributes to cisplatin resistance in non-small cell lung cancer by regulating miR-140-5p/Wnt/β-catenin pathway. Neoplasma. 2019; 66(5):756-765. DOI: 10.4149/neo_2018_181218N980. View

18.

Yu W, Ding J, He M, Chen Y, Wang R, Han Z . Estrogen receptor β promotes the vasculogenic mimicry (VM) and cell invasion via altering the lncRNA-MALAT1/miR-145-5p/NEDD9 signals in lung cancer. Oncogene. 2018; 38(8):1225-1238. DOI: 10.1038/s41388-018-0463-1. View

19.

Liu X, Liu Z, He H, Zhang C, Wang Y . MicroRNA-101-3p suppresses cell proliferation, invasion and enhances chemotherapeutic sensitivity in salivary gland adenoid cystic carcinoma by targeting Pim-1. Am J Cancer Res. 2015; 5(10):3015-29. PMC: 4656727. View

20.

Wang X, Lan Z, He J, Lai Q, Yao X, Li Q . LncRNA SNHG6 promotes chemoresistance through ULK1-induced autophagy by sponging miR-26a-5p in colorectal cancer cells. Cancer Cell Int. 2019; 19:234. PMC: 6734319. DOI: 10.1186/s12935-019-0951-6. View