Cellular Metabolic Activity Marker Via Selective Turn-ON Detection of Transporter Protein Using Nitrobenzoxadiazole-based Fluorescent Reporter

Overview

Journal Sci Rep

Specialty Science

Date 2020 Mar 7

PMID 32139799

Citations 5

Authors

Tanoy Dutta

Kaushik Pal

Apurba L Koner

Affiliations

Soon will be listed here.

Abstract

A nitrobenzoxadiazole-based fluoroprobe (NBD-Bu) is designed to probe cellular metabolic activity in cancer and normal cells. NBD-Bu shows a significant fluorescence enhancement upon selective binding to the transport protein serum albumin in PBS buffer at ambient conditions. Encouraged by this finding, the site- specificity of NBD-Bu has been explored through a competitive displacement assay in the presence of site-specific markers such as warfarin and ibuprofen. Notably, even at micromolar concentrations, the probe possesses the ability to displace the site marker drug ibuprofen, efficiently. Subsequently, high-resolution fluorescence imaging results consolidated the potential of NBD-Bu for detection of abnormal cellular metabolic activity.

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