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Effects of Gamma-Radiation on DNA Damage in Onion (Allium Cepa L.) Seedlings

Overview
Journal Dokl Biochem Biophys
Specialties Biochemistry
Biophysics
Date 2020 Mar 5
PMID 32130600
Citations 1
Authors
A Ya Bolsunovsky
D V Dementyev
T S Frolova
E A Trofimova
E M Iniatkina
S A Vasilyev
O I Sinitsyna
Affiliations
Soon will be listed here.
Abstract

The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.

Citing Articles

Effect of Gamma Radiation on Cytogenetic and Growth Endpoints of Allium cepa Seedlings in Long-Term Experiments.

Bolsunovsky A, Trofimova E, Zueva A, Dementyev D, Petrichenkov M Dokl Biochem Biophys. 2022; 503(1):85-89.

PMID: 35538284 PMC: 9090888. DOI: 10.1134/S1607672922020028.

References
1.
Bolsunovsky A, Dementyev D, Trofimova E, Zotina T . New data on the effect of ionizing radiation on the growth of the aquatic plant Elodea canadensis in the laboratory. Dokl Biol Sci. 2017; 475(1):157-160. DOI: 10.1134/S0012496617040019. View
2.
Olive P . DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int J Radiat Biol. 2001; 75(4):395-405. DOI: 10.1080/095530099140311. View
3.
Collins A . The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol. 2004; 26(3):249-61. DOI: 10.1385/MB:26:3:249. View
4.
Torudd J, Protopopova M, Sarimov R, Nygren J, Eriksson S, Markova E . Dose-response for radiation-induced apoptosis, residual 53BP1 foci and DNA-loop relaxation in human lymphocytes. Int J Radiat Biol. 2005; 81(2):125-38. DOI: 10.1080/09553000500077211. View
5.
Ryu T, Kim J, Kim J, Kim J . Transcriptome-based biological dosimetry of gamma radiation in Arabidopsis using DNA damage response genes. J Environ Radioact. 2017; 181:94-101. DOI: 10.1016/j.jenvrad.2017.11.007. View