Myrrh Oil Inhibitory Growth on Bovine and Equine Piroplasm Parasites and of Mice

Overview

Journal Pathogens

Date 2020 Mar 4

PMID 32121352

Citations 10

Authors

Mahmoud AbouLaila

Shimaa Abd El-Salam El-Sayed

Mosaab A Omar

Mohammad Saleh Al-Aboody

Amer R Abdel Aziz

Mohamed M Abdel-Daim

Mohamed Abdo Rizk

Ikuo Igarashi

Affiliations

Soon will be listed here.

Abstract

The present experimental study was conducted for the assessment of the efficacy of in vitro inhibition of myrrh oil on the propagation of Babesia bovis, B. divergens, B. bigemina, Theileria equi, and B. caballi and in vivo efficacy on B. microti in mice through fluorescence assay based on SYBR green I. The culture of B. divergens B. bovis and was used to evaluate the in vitro possible interaction between myrrh oil and other commercial compound, such as pyronaridine tetraphosphate (PYR), diminazene aceturate (DA), or luteolin. Nested-polymerase chain reaction protocol using primers of the small-subunit rRNA of B. microti was employed to detect any remnants of DNA for studied parasitic species either in blood or tissues. Results elucidated that; Myrrh oil significantly inhibit the growth at 1% of parasitic blood level for all bovine and equine piroplasm under the study. Parasitic regrowth was inhibited subsequently by viability test at 2 µg/mL for B. bigemina and B. bovis, and there was a significant improvement in the in vitro growth inhibition by myrrh oil when combined with DA, PYR, and luteolin. At the same time; mice treated with a combination of myrrh oil/DA showed a higher inhibition in emitted fluorescence signals than the group that challenged with 25 mg/kg of diminazene aceturate at 10 and 12 days post-infection. In conclusion, this study has recommended the myrrh oil to treat animal piroplasmosis, especially in combination with low doses of DA.

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References

Haridy F, Dawoud H, Morsy T . Efficacy of Commiphora molmol (Mirazid) against sheep naturally infected with monieziasis expansa in Al-Santa Center, Gharbia Governorate, Egypt. J Egypt Soc Parasitol. 2004; 34(3):775-82. View

Fujisaki R, Kamei K, Yamamura M, Nishiya H, Inouye S, Takahashi M . In vitro and in vivo anti-plasmodial activity of essential oils, including hinokitiol. Southeast Asian J Trop Med Public Health. 2012; 43(2):270-9. View

El-Sayed S, Rizk M, Terkawi M, Yokoyama N, Igarashi I . Molecular identification and antigenic characterization of Babesia divergens Erythrocyte Binding Protein (BdEBP) as a potential vaccine candidate. Parasitol Int. 2017; 66(6):721-726. DOI: 10.1016/j.parint.2017.07.004. View

Rizk M, El-Sayed S, AbouLaila M, Tuvshintulga B, Yokoyama N, Igarashi I . Large-scale drug screening against Babesia divergens parasite using a fluorescence-based high-throughput screening assay. Vet Parasitol. 2016; 227:93-7. DOI: 10.1016/j.vetpar.2016.07.032. View

Rizk M, El-Sayed S, AbouLaila M, Yokoyama N, Igarashi I . Evaluation of the inhibitory effect of N-acetyl-L-cysteine on Babesia and Theileria parasites. Exp Parasitol. 2017; 179:43-48. DOI: 10.1016/j.exppara.2017.06.003. View

Dolara P, Luceri C, Ghelardini C, Monserrat C, Aiolli S, Luceri F . Analgesic effects of myrrh. Nature. 1996; 379(6560):29. DOI: 10.1038/379029a0. View

Uilenberg G . Babesia--a historical overview. Vet Parasitol. 2006; 138(1-2):3-10. DOI: 10.1016/j.vetpar.2006.01.035. View

Chen Y, Zhou C, Ge Z, Liu Y, Liu Y, Feng W . Composition and potential anticancer activities of essential oils obtained from myrrh and frankincense. Oncol Lett. 2013; 6(4):1140-1146. PMC: 3796379. DOI: 10.3892/ol.2013.1520. View

Soliman O, El-Arman M, Abdul-Samie E, El-Nemr H, Massoud A . Evaluation of myrrh (Mirazid) therapy in fascioliasis and intestinal schistosomiasis in children: immunological and parasitological study. J Egypt Soc Parasitol. 2004; 34(3):941-66. View

10.

Bork S, Yokoyama N, Ikehara Y, Kumar S, Sugimoto C, Igarashi I . Growth-inhibitory effect of heparin on Babesia parasites. Antimicrob Agents Chemother. 2003; 48(1):236-41. PMC: 310193. DOI: 10.1128/AAC.48.1.236-241.2004. View

11.

Vannier E, Gewurz B, Krause P . Human babesiosis. Infect Dis Clin North Am. 2008; 22(3):469-88, viii-ix. PMC: 3998201. DOI: 10.1016/j.idc.2008.03.010. View

12.

Bork S, Yokoyama N, Matsuo T, Claveria F, Fujisaki K, Igarashi I . Growth inhibitory effect of triclosan on equine and bovine Babesia parasites. Am J Trop Med Hyg. 2003; 68(3):334-40. View

13.

Rizk M, El-Sayed S, El-Khodery S, Yokoyama N, Igarashi I . Discovering the in vitro potent inhibitors against Babesia and Theileria parasites by repurposing the Malaria Box: A review. Vet Parasitol. 2019; 274:108895. DOI: 10.1016/j.vetpar.2019.07.003. View

14.

Aboulaila M, Yokoyama N, Igarashi I . Inhibitory effects of (-)-epigallocatechin-3-gallate from green tea on the growth of Babesia parasites. Parasitology. 2009; 137(5):785-91. DOI: 10.1017/S0031182009991594. View

15.

Igarashi I, Njonge F, Kaneko Y, Nakamura Y . Babesia bigemina: in vitro and in vivo effects of curdlan sulfate on growth of parasites. Exp Parasitol. 1998; 90(3):290-3. DOI: 10.1006/expr.1998.4331. View

16.

Al-Mathal E, Fouad M . Myrrh (Commiphora molmol) in treatment of human and sheep dicrocoeliasis dendriticum in Saudi Arabia. J Egypt Soc Parasitol. 2004; 34(2):713-20. View

17.

Rizk M, Ji S, Liu M, El-Sayed S, Li Y, Byamukama B . Closing the empty anti-Babesia gibsoni drug pipeline in vitro using fluorescence-based high throughput screening assay. Parasitol Int. 2020; 75:102054. DOI: 10.1016/j.parint.2020.102054. View

18.

Ganchimeg D, Batbold B, Murata T, Davaapurev B, Munkhjargal T, Tuvshintulga B . Flavonoids isolated from the flowers of Pulsatilla flavescens and their anti-piroplasm activity. J Nat Med. 2019; 73(3):633-640. DOI: 10.1007/s11418-019-01294-8. View

19.

AbouLaila M, Sivakumar T, Yokoyama N, Igarashi I . Inhibitory effect of terpene nerolidol on the growth of Babesia parasites. Parasitol Int. 2010; 59(2):278-82. DOI: 10.1016/j.parint.2010.02.006. View

20.

Guswanto A, Nugraha A, Tuvshintulga B, Tayebwa D, Rizk M, El-Saber Batiha G . 17-DMAG inhibits the multiplication of several Babesia species and Theileria equi on in vitro cultures, and Babesia microti in mice. Int J Parasitol Drugs Drug Resist. 2018; 8(1):104-111. PMC: 6114103. DOI: 10.1016/j.ijpddr.2018.02.005. View