Retrospective Screening and Analysis of and in Gram-Negative Bacteria in China, 2010-2019

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2020 Mar 3

PMID 32117144

Citations 16

Authors

Rong Fan

Chuchu Li

Ran Duan

Shuai Qin

Junrong Liang

Meng Xiao

Dongyue Lv

Huaiqi Jing

Xin Wang

Affiliations

Soon will be listed here.

Abstract

Currently, Gram-negative bacteria have developed multidrug and broad-spectrum drug resistance, and the numbers of species and strains carrying or genes are increasing. In this study, and distribution of 12,858 Gram-negative bacteria isolated from wildlife, patients, livestock, poultry and environment in 14 provinces of China from 2010 to 2019 and the antibiotics resistance in regard to polymyxins (polymyxin B and colistin) and carbapenems of positive strains were investigated. A total of 70 strains of 10 species carried the gene, positive rates of patients, livestock and poultry, and environmental strains were 0.62% (36/5,828), 4.07% (29/712), 5.43% (5/92), respectively. Six strains of 3 species carrying the gene all came from patients 0.10% (6/5,828). Two new gene variants (GenBank: MK965883, MK965884) were identified, one of which contains premature stop codon. The drug susceptibility results showed that all carriers were sensitive to carbapenems, among which, 66 strains were resistant and 4 were sensitive to polymyxins. The strains with the gene had different degrees of resistance to carbapenems and were sensitive to polymyxins. The findings that species carrying or genes were limited and mostly normal flora of opportunistic or low pathogenic organisms indicated that transfer of and genes between bacteria was relatively limited in China. The none detection among wildlife compared with other sources supports the speculation that the emergence of and increase in polymyxins and carbapenem-resistant strains was mainly related to the selective pressure of antibiotics.

Citing Articles

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References

Zenati K, Touati A, Bakour S, Sahli F, Rolain J . Characterization of NDM-1- and OXA-23-producing Acinetobacter baumannii isolates from inanimate surfaces in a hospital environment in Algeria. J Hosp Infect. 2015; 92(1):19-26. DOI: 10.1016/j.jhin.2015.09.020. View

Liassine N, Assouvie L, Descombes M, Denervaud Tendon V, Kieffer N, Poirel L . Very low prevalence of MCR-1/MCR-2 plasmid-mediated colistin resistance in urinary tract Enterobacteriaceae in Switzerland. Int J Infect Dis. 2016; 51:4-5. DOI: 10.1016/j.ijid.2016.08.008. View

Madec J, Haenni M, Nordmann P, Poirel L . Extended-spectrum β-lactamase/AmpC- and carbapenemase-producing Enterobacteriaceae in animals: a threat for humans?. Clin Microbiol Infect. 2017; 23(11):826-833. DOI: 10.1016/j.cmi.2017.01.013. View

Liu B, Song F, Zou M, Hao Z, Shan H . Emergence of Colistin Resistance Gene mcr-1 in Cronobacter sakazakii Producing NDM-9 and in Escherichia coli from the Same Animal. Antimicrob Agents Chemother. 2016; 61(2). PMC: 5278688. DOI: 10.1128/AAC.01444-16. View

Lopez C, Ayala J, Bonomo R, Gonzalez L, Vila A . Protein determinants of dissemination and host specificity of metallo-β-lactamases. Nat Commun. 2019; 10(1):3617. PMC: 6689000. DOI: 10.1038/s41467-019-11615-w. View

Haenni M, Poirel L, Kieffer N, Chatre P, Saras E, Metayer V . Co-occurrence of extended spectrum β lactamase and MCR-1 encoding genes on plasmids. Lancet Infect Dis. 2016; 16(3):281-2. DOI: 10.1016/S1473-3099(16)00007-4. View

Xavier B, Lammens C, Ruhal R, Kumar-Singh S, Butaye P, Goossens H . Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016. Euro Surveill. 2016; 21(27). DOI: 10.2807/1560-7917.ES.2016.21.27.30280. View

Hasman H, Hammerum A, Hansen F, Hendriksen R, Olesen B, Agerso Y . Detection of mcr-1 encoding plasmid-mediated colistin-resistant Escherichia coli isolates from human bloodstream infection and imported chicken meat, Denmark 2015. Euro Surveill. 2015; 20(49). DOI: 10.2807/1560-7917.ES.2015.20.49.30085. View

Alos J . [Antibiotic resistance: A global crisis]. Enferm Infecc Microbiol Clin. 2014; 33(10):692-9. DOI: 10.1016/j.eimc.2014.10.004. View

10.

Chavda B, Lv J, Hou M, Chavda K, Kreiswirth B, Feng Y . Coidentification of and in a Clinical Enterobacter cloacae Isolate from China. Antimicrob Agents Chemother. 2018; 62(10). PMC: 6153785. DOI: 10.1128/AAC.00649-18. View

11.

Liu L, Feng Y, McNally A, Zong Z . blaNDM-21, a new variant of blaNDM in an Escherichia coli clinical isolate carrying blaCTX-M-55 and rmtB. J Antimicrob Chemother. 2018; 73(9):2336-2339. DOI: 10.1093/jac/dky226. View

12.

Brenner Michael G, Freitag C, Wendlandt S, Eidam C, Fessler A, Lopes G . Emerging issues in antimicrobial resistance of bacteria from food-producing animals. Future Microbiol. 2015; 10(3):427-43. DOI: 10.2217/fmb.14.93. View

13.

Hadjadj L, Riziki T, Zhu Y, Li J, Diene S, Rolain J . Study of mcr-1 Gene-Mediated Colistin Resistance in Enterobacteriaceae Isolated from Humans and Animals in Different Countries. Genes (Basel). 2017; 8(12). PMC: 5748712. DOI: 10.3390/genes8120394. View

14.

Kumarasamy K, Toleman M, Walsh T, Bagaria J, Butt F, Balakrishnan R . Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect Dis. 2010; 10(9):597-602. PMC: 2933358. DOI: 10.1016/S1473-3099(10)70143-2. View

15.

Kong L, Lei C, Ma S, Jiang W, Liu B, Wang Y . Various Sequence Types of Escherichia coli Isolates Coharboring and Genes from a Commercial Swine Farm in China. Antimicrob Agents Chemother. 2016; 61(3). PMC: 5328568. DOI: 10.1128/AAC.02167-16. View

16.

Pitout J, Nordmann P, Poirel L . Carbapenemase-Producing Klebsiella pneumoniae, a Key Pathogen Set for Global Nosocomial Dominance. Antimicrob Agents Chemother. 2015; 59(10):5873-84. PMC: 4576115. DOI: 10.1128/AAC.01019-15. View

17.

Spalding Walters M, Eggers P, Albrecht V, Travis T, Lonsway D, Hovan G . Vancomycin-Resistant Staphylococcus aureus - Delaware, 2015. MMWR Morb Mortal Wkly Rep. 2015; 64(37):1056. DOI: 10.15585/mmwr.mm6437a6. View

18.

Fernandes M, Moura Q, Sartori L, Silva K, Cunha M, Esposito F . Silent dissemination of colistin-resistant Escherichia coli in South America could contribute to the global spread of the mcr-1 gene. Euro Surveill. 2016; 21(17). DOI: 10.2807/1560-7917.ES.2016.21.17.30214. View

19.

Carattoli A . Plasmids and the spread of resistance. Int J Med Microbiol. 2013; 303(6-7):298-304. DOI: 10.1016/j.ijmm.2013.02.001. View

20.

Zhao F, Feng Y, Lu X, McNally A, Zong Z . IncP Plasmid Carrying Colistin Resistance Gene mcr-1 in Klebsiella pneumoniae from Hospital Sewage. Antimicrob Agents Chemother. 2016; 61(2). PMC: 5278755. DOI: 10.1128/AAC.02229-16. View