Determination of the Infectivity of Cryopreserved Sporozoites in Tick Derived Stabilates Iran Ak-93 Strain, by In Vivo and In Vitro Methods

Overview

Journal Iran J Parasitol

Publisher Tehran University of Medical Sciences

Specialty Parasitology

Date 2020 Feb 27

PMID 32099560

Citations 2

Authors

Hossein Modirrousta

Gholamreza Habibi

Parviz Shayan

Asghar Afshari

Ali Mirjalili

Mohamad Abdigoudarzi

Affiliations

Soon will be listed here.

Abstract

Background: The protozoan parasite is the causative agent of tropical theileriosis in cattle. Vaccination is recommended by administration of attenuated schizont-infected cell lines. The expected protective immunity post-vaccination can be demonstrated by challenge test through inoculation of highly virulent infective sporozoites. The aim of this study was to produce tick infected with (local strain) for preparation of tick-derived sporozoite stabilates for molecular characterization and infectivity test assay.

Methods: A local strain was used for experimental infection of calves. A field isolate of was isolated, laboratory-reared and infected by blood-feeding on infected above-mentioned calves. The infectivity of calf, tick and prepared stabilate were confirmed by clinical signs of theileriosis, microscopic inspection, RT-PCR and in vitro cell culture.

Results: The tick stabilate was prepared and cryopreserved in liquid nitrogen. The infectivity of the tick stabilate was verified by in vivo bioassay, in vitro cell culture infection, microscopic inspection in salivary glands and RT-PCR assay. The in vitro produced cell line in this study was characterized by Cytochrome b gene analyzing.

Conclusion: The infectivity of a new prepared tick-derived sporozoite stabilate was confirmed in susceptible calves; by microscopically, post mortem, tick microscopic and molecular assays. Moreover, naïve PBMCs were transformed and proliferated by infected tick stabilate to immortal schizont infected cell line. The potent infective sporozoite tick derived stabilate could be used for vaccine efficacy and challenge test as well as in vaccine development.

Citing Articles

Development of Low-Serum Culture Media for the in Vitro Cultivation of S15 Cell Line.

Habibi G, Afshari A, Shahedi A, Hashemlou M, Es-Haghi A, Mehrabadi M Iran J Parasitol. 2024; 19(1):75-85.

PMID: 38654949 PMC: 11033534. DOI: 10.18502/ijpa.v19i1.15206.

Establishment and Validation of Sporozoite Ak-93 Infection in Laboratory-Reared Tick Using In Vivo and In Vitro Assays.

Afshari A, Habibi G, Abdigoudarzi M, Yazdani F J Arthropod Borne Dis. 2021; 14(3):261-269.

PMID: 33644239 PMC: 7903359. DOI: 10.18502/jad.v14i3.4559.

References

Singh D, Jagdish S, Gautam O, Dhar S . Infectivity of ground-up tick supernates prepared from Theilerai annulata infected Hyalomma anatolicum anatolicum. Trop Anim Health Prod. 1979; 11(2):87-90. DOI: 10.1007/BF02237776. View

Cunningham M, Brown C, Burridge M, Purnell R . Cryopreservation of infective particles of Theileria parva. Int J Parasitol. 1973; 3(5):583-7. DOI: 10.1016/0020-7519(73)90082-9. View

Marcotty T, Speybroeck N, Berkvens D, Chaka G, Besa R, Madder M . In vitro titration of Theileria parva tick derived stabilates. Parasitology. 2004; 128(Pt 2):131-7. DOI: 10.1017/s0031182003004323. View

Biglari P, Bakhshi H, Chinikar S, Belqeiszadeh H, Ghaffari M, Javaherizadeh S . as the Main Infesting Tick in an Important Livestock Rearing Region, Central Area of Iran. Iran J Public Health. 2018; 47(5):742-749. PMC: 6005964. View

Ouhelli H, Innes E, Brown C, Walker A, Simpson S, SPOONER R . The effect of dose and line on immunisation of cattle with lymphoblastoid cells infected with Theileria annulata. Vet Parasitol. 1989; 31(3-4):217-28. DOI: 10.1016/0304-4017(89)90071-x. View

Gill B, Bhattacharyulu Y, Kaur D, Singh A . Immunisation of cattle against tropical theileriosis (Theileria annulata infection) by "infection-treatment" method. Ann Rech Vet. 1977; 8(3):285-92. View

SPOONER R, Innes E, Glass E, Millar P, Brown C . Bovine mononuclear cell lines transformed by Theileria parva or Theileria annulata express different subpopulation markers. Parasite Immunol. 1988; 10(6):619-29. DOI: 10.1111/j.1365-3024.1988.tb00249.x. View

Babiuk L . Vaccination: a management tool in veterinary medicine. Vet J. 2002; 164(3):188-201. DOI: 10.1053/tvjl.2001.0663. View

Arjmand Yamchi J, Tavassoli M . Survey on infection rate, vectors and molecular identification of Theileria annulata in cattle from North West, Iran. J Parasit Dis. 2016; 40(3):1071-6. PMC: 4996249. DOI: 10.1007/s12639-014-0636-y. View

10.

Flach E, Ouhelli H, Waddington D, El Hasnaoui M . Prevalence of Theileria in the tick Hyalomma detritum detritum in the Doukkala region, Morocco. Med Vet Entomol. 1993; 7(4):343-50. DOI: 10.1111/j.1365-2915.1993.tb00703.x. View

11.

Aktas M, Dumanli N, Angin M . Cattle infestation by Hyalomma ticks and prevalence of Theileria in Hyalomma species in the east of Turkey. Vet Parasitol. 2004; 119(1):1-8. DOI: 10.1016/j.vetpar.2003.10.013. View

12.

Shaw M . Cell invasion by Theileria sporozoites. Trends Parasitol. 2002; 19(1):2-6. DOI: 10.1016/s1471-4922(02)00015-6. View

13.

Haque M, Jyoti , Singh N, Rath S . Prevalence of Theileria annulata infection in Hyalomma anatolicum anatolicum in Punjab state, India. J Parasit Dis. 2011; 34(1):48-51. PMC: 3081700. DOI: 10.1007/s12639-010-0004-5. View

14.

Samish M, Pipano E . Transmission of Theileria annulata (Dschunkowsky & Luhs, 1904) by Hyalomma excavatum (Koch, 1844). Parasitology. 1983; 86 (Pt 2):269-74. DOI: 10.1017/s0031182000050435. View

15.

Kurtti T, Munderloh U, Irvin A, Buscher G . Theileria parva: early events in the development of bovine lymphoblastoid cell lines persistently infected with macroschizonts. Exp Parasitol. 1981; 52(2):280-90. DOI: 10.1016/0014-4894(81)90083-7. View