Fourier Transform Infrared Spectroscopy Monitoring of Dihydroartemisinin-Induced Growth Inhibition in Ovarian Cancer Cells and Normal Ovarian Surface Epithelial Cells

Overview

Journal Cancer Manag Res

Publisher Dove Medical Press

Specialty Oncology

Date 2020 Feb 27

PMID 32099462

Citations 5

Authors

Lei Li

Jinguang Wu

Shifu Weng

Limin Yang

Huizi Wang

Yizhuang Xu

Keng Shen

Affiliations

Soon will be listed here.

Abstract

Purpose: Ovarian cancer is the most lethal of gynecological malignancies. Dihydroartemisinin (DHA), a derivative of artemisinin (ARS), has profound effects against human tumors. The aim of this study was to provide a convenient, cost-efficient technique, Fourier transform infrared (FTIR) spectroscopy, to monitor and evaluate responses to DHA-induced growth inhibition of ovarian cancer cells.

Methods: Cell growth and viability and the 50% inhibitory concentration (IC50) of DHA were assessed by the MTT assay. FTIR spectroscopy was used to monitor cells following DHA treatment, and data were analyzed by OMNIC 8.0 software.

Results: DHA can decrease the viability of ovarian cancer cells and normal cells, but cancer cells were more sensitive to this drug than normal cells. Spectral differences were observed between cells with or without DHA treatment. In particular, an increase in the amount of lipids and nucleic acids was observed. The band intensity ratio of 1454/1400, and the intensity of the band 1741 cm increased, indicating stronger absorption after DHA treatment. Moreover, the differences were larger for the cell lines that were more sensitive to DHA.

Conclusion: The spectral features provided information about important molecular characteristics of the cells in response to chemicals. These findings demonstrated the possible use of FTIR spectroscopy to evaluate DHA-induced growth inhibition effects in ovarian cancer cells and provided a promising new tool for monitoring cell growth and the effects of antitumor drugs in the clinic in the future.

Citing Articles

Artemisinin and Its Derivatives as Potential Anticancer Agents.

Wen L, Chan B, Qiu M, Leung P, Wong C Molecules. 2024; 29(16).

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Evaluation of Proteomic and Lipidomic Changes in -Infected Trout Kidney Tissue with the Use of FT-IR Spectroscopy and MALDI Mass Spectrometry Imaging.

Matys J, Turska-Szewczuk A, Gieroba B, Kurzylewska M, Pekala-Safinska A, Sroka-Bartnicka A Int J Mol Sci. 2022; 23(20).

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Dihydroartemisinin: A Potential Drug for the Treatment of Malignancies and Inflammatory Diseases.

Yu R, Jin G, Fujimoto M Front Oncol. 2021; 11:722331.

PMID: 34692496 PMC: 8529146. DOI: 10.3389/fonc.2021.722331.

Dihydroartemisinin-Loaded Chitosan Nanoparticles Inhibit the Rifampicin-Resistant by Disrupting the Cell Wall.

Gu X, Cheng Q, He P, Zhang Y, Jiang Z, Zeng Y Front Microbiol. 2021; 12:735166.

PMID: 34630358 PMC: 8500176. DOI: 10.3389/fmicb.2021.735166.

Fourier Transform Infrared Spectroscopy: An Innovative Method for the Diagnosis of Ovarian Cancer.

Li L, Wu J, Yang L, Wang H, Xu Y, Shen K Cancer Manag Res. 2021; 13:2389-2399.

PMID: 33737836 PMC: 7965685. DOI: 10.2147/CMAR.S291906.

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