Immunogenicity of a New, Inactivated Canine Adenovirus Type 2 Vaccine for Dogs

Overview

Journal Clin Exp Vaccine Res

Date 2020 Feb 26

PMID 32095439

Citations 1

Authors

Dong-Kun Yang

Ha-Hyun Kim

Jae Young Yoo

Miryeon Ji

Bok Hee Han

Subin Oh

Bang-Hun Hyun

Affiliations

Soon will be listed here.

Abstract

Purpose: We constructed a new canine adenovirus type 2 (CAV-2) vaccine candidate using the recently isolated Korean CAV-2 strain; we termed the vaccine APQA1701-40P and evaluated its safety and immunogenicity in dogs.

Materials And Methods: To generate the anti-CAV-2 vaccine, APQA1701 was passaged 40 times in MDCK cells growing in medium containing 5 mM urea and the virus was inactivated using 0.05% (volume per volume) formaldehyde. Two vaccines were prepared by blending inactivated APQA1701-40P with two different adjuvants; both were intramuscularly injected (twice) into guinea pigs. The safety and immunogenicity of the Cabopol-adjuvanted vaccine were evaluated in seronegative dogs. The humoral responses elicited were measured using an indirect enzyme-linked immunosorbent assay (I-ELISA), and via a virus neutralization assay (VNA).

Results: The new, inactivated CAV-2 vaccine strain, APQA1701-40P, lacked six amino acids of the E1b-19K protein. In guinea pigs, the Cabopol-adjuvanted vaccine afforded a slightly higher VNA titer and I-ELISA absorbance than an IMS gel-adjuvanted vaccine 4 weeks post-vaccination (p>0.05). Dogs inoculated with the former vaccine developed a significantly higher immune titer than non-vaccinated dogs.

Conclusion: The Cabopol-adjuvanted, inactivated CAV-2 vaccine was safe and induced a high VNA titer in dogs.

Citing Articles

Immunogenicity of a novel inactivated canine adenovirus type 2 variant vaccine for dogs.

Yang D, Ahn S, Lee H, Kim M, Kim J, Lee J Clin Exp Vaccine Res. 2024; 13(3):253-258.

PMID: 39144129 PMC: 11319112. DOI: 10.7774/cevr.2024.13.3.253.

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