Generation and Evaluation of a Glaesserella (Haemophilus) Parasuis Capsular Mutant

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2020 Feb 26

PMID 32094250

Citations 7

Authors

Kirsten C Eberle

Samantha J Hau

Shi-Lu Luan

Lucy A Weinert

Judith A Stasko

Jinhong Wang

Sarah E Peters

Paul R Langford

Andrew N Rycroft

Brendan W Wren

Duncan J Maskell

Alexander W Tucker

Susan L Brockmeier

Affiliations

Soon will be listed here.

Abstract

() is a commensal bacterium of the upper respiratory tract in pigs and also the causative agent of Glässer's disease, which causes significant morbidity and mortality in pigs worldwide. Isolates are characterized into 15 serovars by their capsular polysaccharide, which has shown a correlation with isolate pathogenicity. To investigate the role the capsule plays in virulence and host interaction, a capsule mutant of the serovar 5 strain HS069 was generated (HS069Δcap) through allelic exchange following natural transformation. HS069Δcap was unable to cause signs of systemic disease during a pig challenge study and had increased sensitivity to complement killing and phagocytosis by alveolar macrophages. Compared with the parent strain, HS069Δcap produced more robust biofilm and adhered equivalently to 3D4/31 cells; however, it was unable to persistently colonize the nasal cavity of inoculated pigs, with all pigs clearing HS069Δcap by 5 days postchallenge. Our results indicate the importance of the capsular polysaccharide to virulence as well as nasal colonization in pigs.

Citing Articles

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