Ultrasound-assisted Hydrolysis of Lard for Free Fatty Acids Catalyzed by Combined Two Lipases in Aqueous Medium

Overview

Journal Bioengineered

Date 2020 Feb 25

PMID 32091302

Citations 7

Authors

Jinjin Huang

Qingyi Zhao

Wei Bu

Chunmei Zhang

Zhen Yang

Xin Zhang

Kaini Zhang

Affiliations

Soon will be listed here.

Abstract

Lard is a by-product of animal processing. It is inexpensive compared with vegetable oils; however, its use is limited due to the high calorific value and high-saturated fatty acid content. While using lard as the source of free fatty acids (FFA) can significantly increase its utilization value. This study aimed to research the method on efficient hydrolysis of lard catalyzed by -lipases and assisted with ultrasound pretreatment. A 1,3-specific lipase from (termed pRML, 1540 U/mL) and a nonspecific mono- and diacylglycerol lipase from (termed MDL, 2000 U/mL) were used as biocatalysts. Results showed that the maximum hydrolysis rate of lard after 6 h at 45°C by using pRML and MDL alone was, respectively, 39.9% and 8.5%. When pRML combined with MDL (-lipases), hydrolysis rate can reach to 78.1%. While -lipases were assisted with 5 min ultrasound pretreatment before the reaction, the hydrolysis rate can further increase to 97%. The -lipases with different specificity and assisted with ultrasound pretreatment may be a useful technology for the enzyme production of FFA from complex lipid substrates, such as lard.

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