Exploring the Therapeutic Composition and Mechanism of Jiang-Suan-Chu-Bi Recipe on Gouty Arthritis Using an Integrated Approach Based on Chemical Profile, Network Pharmacology and Experimental Support Using Molecular Cell Biology

Overview

Journal Front Pharmacol

Date 2020 Feb 22

PMID 32082152

Citations 7

Authors

Nan Xiao

Jialin Qu

Shiyong He

Peng Huang

Yanling Qiao

Guangxing Li

Taowen Pan

Hua Sui

Lin Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Gouty arthritis is a common metabolic disease caused by long-term purine metabolic disorder and elevated serum uric acid. Jiang-Suan-Chu-Bi recipe (JSCBR), a traditional Chinese herbal formula prescribed according to utilization frequency and cluster analysis, has been clinically validated remedy for gouty arthritis. However, its therapeutic composition and mechanism remains unclear.

Methods: In the present study, a simple, rapid, and sensitive ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS)-based chemical profiling was firstly established for comprehensively identifying the major constituents in JSCBR. A phytochemistry-based network pharmacology analysis was further performed to explore the potential therapeutic targets and pathways involved in JSCBR bioactivity. Finally, THP-1 cell model was used to verify the prediction results of network pharmacology by western blot analysis.

Results: A total of 139 compounds containing phenolic acids, flavonoids, triterpenoid saponins, alkaloids, amino acids, fatty acids, anthraquinones, terpenes, coumarins, and other miscellaneous compounds were identified, respectively. 175 disease genes, 51 potential target nodes, 80 compounds, and 11 related pathways based on network pharmacology analysis were achieved. Among these pathways and genes, NOD-like receptor signaling pathway may play an important role in the curative effect of JSCBR on gouty arthritis by regulation of NRLP3/ASC/CASP1/IL1B. The results of cellular and molecular experiments showed that JSCBR can effectively reduce the protein expression of ASC, caspase-1, IL-1β, and NRLP3 in monosodium urate-induced THP-1 cells, which indicated that JSCBR mediated inflammation in gouty arthritis by inhibiting the activation of NOD-like receptor signaling pathway.

Conclusion: Thus, the integrated approaches adopted in the present study could contribute to simplifying the complex system and providing directions for further research of JSCBR.

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References

Li H, Ou G, He Y, Ren L, Yang X, Zeng M . Resveratrol attenuates the MSU crystal-induced inflammatory response through the inhibition of TAK1 activity. Int Immunopharmacol. 2018; 67:62-68. DOI: 10.1016/j.intimp.2018.12.004. View

Wilson L, Saseen J . Gouty Arthritis: A Review of Acute Management and Prevention. Pharmacotherapy. 2016; 36(8):906-22. DOI: 10.1002/phar.1788. View

McCormack W, Parker A, ONeill L . Toll-like receptors and NOD-like receptors in rheumatic diseases. Arthritis Res Ther. 2009; 11(5):243. PMC: 2787278. DOI: 10.1186/ar2729. View

Xu S, Qi X, Liu Y, Liu Y, Lv X, Sun J . UPLC-MS/MS of Atractylenolide I, Atractylenolide II, Atractylenolide III, and Atractyloside A in Rat Plasma after Oral Administration of Raw and Wheat Bran-Processed Atractylodis Rhizoma. Molecules. 2018; 23(12). PMC: 6320938. DOI: 10.3390/molecules23123234. View

Liang J, Xu F, Zhang Y, Huang S, Zang X, Zhao X . The profiling and identification of the absorbed constituents and metabolites of Paeoniae Radix Rubra decoction in rat plasma and urine by the HPLC-DAD-ESI-IT-TOF-MS(n) technique: a novel strategy for the systematic screening and identification of.... J Pharm Biomed Anal. 2013; 83:108-21. DOI: 10.1016/j.jpba.2013.04.029. View

Ma X, Leung A, Chan C, Su T, Li W, Li S . UHPLC UHD Q-TOF MS/MS analysis of the impact of sulfur fumigation on the chemical profile of Codonopsis Radix (Dangshen). Analyst. 2013; 139(2):505-16. DOI: 10.1039/c3an01561k. View

Dong Y, Qiu P, Zhu R, Zhao L, Zhang P, Wang Y . A Combined Phytochemistry and Network Pharmacology Approach to Reveal the Potential Antitumor Effective Substances and Mechanism of . Front Pharmacol. 2019; 10:266. PMC: 6434905. DOI: 10.3389/fphar.2019.00266. View

Pascart T, Grandjean A, Capon B, Legrand J, Namane N, Ducoulombier V . Monosodium urate burden assessed with dual-energy computed tomography predicts the risk of flares in gout: a 12-month observational study : MSU burden and risk of gout flare. Arthritis Res Ther. 2018; 20(1):210. PMC: 6142357. DOI: 10.1186/s13075-018-1714-9. View

He X, Yi T, Tang Y, Xu J, Zhang J, Zhang Y . Assessing the quality of Smilacis Glabrae Rhizoma (Tufuling) by colormetrics and UPLC-Q-TOF-MS. Chin Med. 2016; 11:33. PMC: 4936051. DOI: 10.1186/s13020-016-0104-y. View

10.

Bai Y, Xu J, Kong M, Gao Q, Liu L, Li S . Discovery of characteristic chemical markers for inspecting sulfur-fumigated Radix Angelicae Sinensis by ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry based metabolomics and chemical profiling approach. Food Res Int. 2017; 76(Pt 3):387-394. DOI: 10.1016/j.foodres.2015.05.055. View

11.

Chen L, Tang Y, Chen M, Deng H, Yan X, Wu D . Chemical correlation between Gegen Qinlian dispensing granule and its four raw herbs by LC fingerprint. Phytomedicine. 2010; 17(2):100-7. DOI: 10.1016/j.phymed.2009.10.012. View

12.

Guo L, Li R, Liu K, Yang J, Li H, Li S . Structural characterization and discrimination of Chinese medicinal materials with multiple botanical origins based on metabolite profiling and chemometrics analysis: Clematidis Radix et Rhizoma as a case study. J Chromatogr A. 2015; 1425:129-40. DOI: 10.1016/j.chroma.2015.11.013. View

13.

Ling X, Bochu W . A review of phytotherapy of gout: perspective of new pharmacological treatments. Pharmazie. 2014; 69(4):243-56. View

14.

Ge A, Ma W, Wang C, Li J, He J, Liu E . Ultra high performance liquid chromatography with photodiode array detector and quadrupole time-of-flight tandem mass spectrometry coupled with discriminant analysis to evaluate Angelicae pubescentis radix from different regions. J Sep Sci. 2014; 37(18):2523-34. DOI: 10.1002/jssc.201400289. View

15.

Liu Y, Li L, Xiao Y, Yao J, Li P, Yu D . Global metabolite profiling and diagnostic ion filtering strategy by LC-QTOF MS for rapid identification of raw and processed pieces of Rheum palmatum L. Food Chem. 2015; 192:531-40. DOI: 10.1016/j.foodchem.2015.07.013. View

16.

Jhang J, Lin J, Yen G . Beneficial Properties of Phytochemicals on NLRP3 Inflammasome-Mediated Gout and Complication. J Agric Food Chem. 2018; 66(4):765-772. DOI: 10.1021/acs.jafc.7b05113. View

17.

Sun H, Wang H, Zhang A, Yan G, Han Y, Li Y . Chemical Discrimination of Cortex Phellodendri amurensis and Cortex Phellodendri chinensis by Multivariate Analysis Approach. Pharmacogn Mag. 2016; 12(45):41-9. PMC: 4787336. DOI: 10.4103/0973-1296.176023. View

18.

Huang P, Ke H, Qiu Y, Cai M, Qu J, Leng A . Systematically Characterizing Chemical Profile and Potential Mechanisms of Qingre Lidan Decoction Acting on Cholelithiasis by Integrating UHPLC-QTOF-MS and Network Target Analysis. Evid Based Complement Alternat Med. 2019; 2019:2675287. PMC: 6335670. DOI: 10.1155/2019/2675287. View

19.

Wang X, Qin Y, Li G, Chen S, Ma J, Guo Y . Study on Chemical Constituents in Polygoni Cuspidati Folium and its Preparation by UPLC-ESI-Q-TOF-MS/MS. J Chromatogr Sci. 2018; 56(5):425-435. DOI: 10.1093/chromsci/bmy017. View

20.

Zhou Q, Lin F, Liu S, Sui X . Influence of the total saponin fraction from Dioscorea nipponica Makino on TLR2/4-IL1R receptor singnal pathway in rats of gouty arthritis. J Ethnopharmacol. 2017; 206:274-282. DOI: 10.1016/j.jep.2017.04.024. View