Virus Isolation from the First Patient with SARS-CoV-2 in Korea

Overview

Journal J Korean Med Sci

Specialty General Medicine

Date 2020 Feb 22

PMID 32080990

Citations 122

Authors

Wan Beom Park

Nak Jung Kwon

Su Jin Choi

Chang Kyung Kang

Pyoeng Gyun Choe

Jin Yong Kim

Jiyoung Yun

Gir Won Lee

Moon Woo Seong

Nam Joong Kim

Jeong Sun Seo

Myoung Don Oh

Affiliations

Soon will be listed here.

Abstract

Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.

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