Regulation of Electromagnetic Perceptive Gene Using Ferromagnetic Particles for the External Control of Calcium Ion Transport

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2020 Feb 21

PMID 32075263

Citations 16

Authors

Jangsun Hwang

Yonghyun Choi

Kyungwoo Lee

Vijai Krishnan

Galit Pelled

Assaf A Gilad

Jonghoon Choi

Affiliations

Soon will be listed here.

Abstract

Developing synthetic biological devices to allow the noninvasive control of cell fate and function, in vivo can potentially revolutionize the field of regenerative medicine. To address this unmet need, we designed an artificial biological "switch" that consists of two parts: (1) the electromagnetic perceptive gene (EPG) and (2) magnetic particles. Our group has recently cloned the EPG from the (glass catfish). The EPG gene encodes a putative membrane-associated protein that responds to electromagnetic fields (EMFs). This gene's primary mechanism of action is to raise the intracellular calcium levels or change in flux through EMF stimulation. Here, we developed a system for the remote regulation of [Ca] (i.e., intracellular calcium ion concentration) using streptavidin-coated ferromagnetic particles (FMPs) under a magnetic field. The results demonstrated that the EPG-FMPs can be used as a molecular calcium switch to express target proteins. This technology has the potential for controlled gene expression, drug delivery, and drug developments.

Citing Articles

Magnetogenetic stimulation inside MRI induces spontaneous and evoked changes in neural circuits activity in rats.

Chuang K, Qian C, Gilad A, Pelled G Front Neurosci. 2024; 18:1459120.

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A conserved phenylalanine motif among teleost fish provides insight for improving electromagnetic perception.

Ricker B, Castellanos Franco E, de Los Campos G, Pelled G, Gilad A Open Biol. 2024; 14(7):240092.

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A conserved phenylalanine motif among Teleost fish provides insight for improving electromagnetic perception.

Ricker B, Castellanos Franco E, de Los Campos G, Pelled G, Gilad A bioRxiv. 2024; .

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Grady C, Castellanos Franco E, Schossau J, Ashbaugh R, Pelled G, Gilad A Front Bioeng Biotechnol. 2024; 12:1355915.

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