Sub-lethal Concentrations of Phytochemicals (Carvacrol and Oregano) Select for Reduced Susceptibility Mutants of O23:H52

Overview

Journal Pol J Microbiol

Specialty Microbiology

Date 2020 Feb 19

PMID 32067440

Citations 7

Authors

Afnan A Al-Mnaser

Martin J Woodward

Affiliations

Soon will be listed here.

Abstract

studies aimed at studying the mechanism of action of carvacrol and oregano as natural anti-bacterial agents to control multiple antibiotic-resistant avian pathogenic (APEC) strain O23:H52 isolated from chicken were performed. Derivatives with increased minimum inhibitory concentrations (MIC) to the phytochemicals were selected after growing () strain O23:H52 at sub-lethal concentrations of carvacrol and oregano for a period of 60 days. Whole-genome sequencing (WGS) of two derivatives revealed a missense mutation in and : the genes responsible for survival mechanisms and antibiotic resistance by efflux, respectively. studies aimed at studying the mechanism of action of carvacrol and oregano as natural anti-bacterial agents to control multiple antibiotic-resistant avian pathogenic (APEC) strain O23:H52 isolated from chicken were performed. Derivatives with increased minimum inhibitory concentrations (MIC) to the phytochemicals were selected after growing () strain O23:H52 at sub-lethal concentrations of carvacrol and oregano for a period of 60 days. Whole-genome sequencing (WGS) of two derivatives revealed a missense mutation in and : the genes responsible for survival mechanisms and antibiotic resistance by efflux, respectively.

Citing Articles

A critical role for iron and zinc homeostatic systems in the evolutionary adaptation of to metal restriction.

Paterson J, Wadsworth J, Hu P, Sharples G Microb Genom. 2023; 9(12).

PMID: 38054971 PMC: 10763504. DOI: 10.1099/mgen.0.001153.

A Critical Review of AMR Risks Arising as a Consequence of Using Biocides and Certain Metals in Food Animal Production.

James C, James S, Onarinde B, Dixon R, Williams N Antibiotics (Basel). 2023; 12(11).

PMID: 37998771 PMC: 10668721. DOI: 10.3390/antibiotics12111569.

Carvacrol Selective Pressure Allows the Occurrence of Genetic Resistant Variants of EGD-e.

Berdejo D, Gayan E, Pagan E, Merino N, Campillo R, Pagan R Foods. 2023; 11(20).

PMID: 37431028 PMC: 9602272. DOI: 10.3390/foods11203282.

Bactericidal Activity of Carvacrol against Involves Alteration of Membrane Fluidity and Integrity through Interaction with Membrane Phospholipids.

Wijesundara N, Lee S, Cheng Z, Davidson R, Langelaan D, Rupasinghe H Pharmaceutics. 2022; 14(10).

PMID: 36297428 PMC: 9607399. DOI: 10.3390/pharmaceutics14101992.

Polyphenolic phytochemicals as natural feed additives to control bacterial pathogens in the chicken gut.

Al-Mnaser A, Dakheel M, Alkandari F, Woodward M Arch Microbiol. 2022; 204(5):253.

PMID: 35412092 PMC: 9001821. DOI: 10.1007/s00203-022-02862-5.

References

Sulavik M, Gambino L, Miller P . The MarR repressor of the multiple antibiotic resistance (mar) operon in Escherichia coli: prototypic member of a family of bacterial regulatory proteins involved in sensing phenolic compounds. Mol Med. 1995; 1(4):436-46. PMC: 2230000. View

Soksawatmaekhin W, Kuraishi A, Sakata K, Kashiwagi K, Igarashi K . Excretion and uptake of cadaverine by CadB and its physiological functions in Escherichia coli. Mol Microbiol. 2004; 51(5):1401-12. DOI: 10.1046/j.1365-2958.2003.03913.x. View

Miladi H, Zmantar T, Chaabouni Y, Fedhila K, Bakhrouf A, Mahdouani K . Antibacterial and efflux pump inhibitors of thymol and carvacrol against food-borne pathogens. Microb Pathog. 2016; 99:95-100. DOI: 10.1016/j.micpath.2016.08.008. View

Bryant J, Chewapreecha C, Bentley S . Developing insights into the mechanisms of evolution of bacterial pathogens from whole-genome sequences. Future Microbiol. 2012; 7(11):1283-1296. PMC: 3996552. DOI: 10.2217/fmb.12.108. View

Rajamanickam K, Yang J, Sakharkar M . Phytochemicals as alternatives to antibiotics against major pathogens involved in bovine respiratory disease(BRD)and bovine mastitis(BM). Bioinformation. 2019; 15(1):32-35. PMC: 6651029. DOI: 10.6026/97320630015032. View

Wong J, de Souza R, Kendall C, Emam A, Jenkins D . Colonic health: fermentation and short chain fatty acids. J Clin Gastroenterol. 2006; 40(3):235-43. DOI: 10.1097/00004836-200603000-00015. View

Joensen K, Tetzschner A, Iguchi A, Aarestrup F, Scheutz F . Rapid and Easy In Silico Serotyping of Escherichia coli Isolates by Use of Whole-Genome Sequencing Data. J Clin Microbiol. 2015; 53(8):2410-26. PMC: 4508402. DOI: 10.1128/JCM.00008-15. View

George A, Levy S . Amplifiable resistance to tetracycline, chloramphenicol, and other antibiotics in Escherichia coli: involvement of a non-plasmid-determined efflux of tetracycline. J Bacteriol. 1983; 155(2):531-40. PMC: 217720. DOI: 10.1128/jb.155.2.531-540.1983. View

Tetsch L, Koller C, Donhofer A, Jung K . Detection and function of an intramolecular disulfide bond in the pH-responsive CadC of Escherichia coli. BMC Microbiol. 2011; 11:74. PMC: 3096576. DOI: 10.1186/1471-2180-11-74. View

10.

Luz I, de Melo A, Bezerra T, Madruga M, Magnani M, de Souza E . Sublethal amounts of Origanum vulgare L. essential oil and carvacrol cause injury and changes in membrane fatty acid of Salmonella Typhimurium cultivated in a meat broth. Foodborne Pathog Dis. 2014; 11(5):357-61. DOI: 10.1089/fpd.2013.1695. View

11.

Tetsch L, Koller C, Haneburger I, Jung K . The membrane-integrated transcriptional activator CadC of Escherichia coli senses lysine indirectly via the interaction with the lysine permease LysP. Mol Microbiol. 2007; 67(3):570-83. DOI: 10.1111/j.1365-2958.2007.06070.x. View

12.

Sikkema J, de Bont J, Poolman B . Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev. 1995; 59(2):201-22. PMC: 239360. DOI: 10.1128/mr.59.2.201-222.1995. View

13.

Friedman M, Henika P, Mandrell R . Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. J Food Prot. 2002; 65(10):1545-60. DOI: 10.4315/0362-028x-65.10.1545. View

14.

Ultee A, Bennik M, Moezelaar R . The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Appl Environ Microbiol. 2002; 68(4):1561-8. PMC: 123826. DOI: 10.1128/AEM.68.4.1561-1568.2002. View

15.

Cristani M, DArrigo M, Mandalari G, Castelli F, Sarpietro M, Micieli D . Interaction of four monoterpenes contained in essential oils with model membranes: implications for their antibacterial activity. J Agric Food Chem. 2007; 55(15):6300-8. DOI: 10.1021/jf070094x. View

16.

Sepehri S, Kotlowski R, Bernstein C, Krause D . Phylogenetic analysis of inflammatory bowel disease associated Escherichia coli and the fimH virulence determinant. Inflamm Bowel Dis. 2009; 15(11):1737-45. DOI: 10.1002/ibd.20966. View

17.

Skalicka-Wozniak K, Walasek M, Aljarba T, Stapleton P, Gibbons S, Xiao J . The anticonvulsant and anti-plasmid conjugation potential of Thymus vulgaris chemistry: An in vivo murine and in vitro study. Food Chem Toxicol. 2018; 120:472-478. DOI: 10.1016/j.fct.2018.07.045. View

18.

Watson N, Dunyak D, Rosey E, Slonczewski J, Olson E . Identification of elements involved in transcriptional regulation of the Escherichia coli cad operon by external pH. J Bacteriol. 1992; 174(2):530-40. PMC: 205747. DOI: 10.1128/jb.174.2.530-540.1992. View

19.

Chueca B, Renzoni A, Berdejo D, Pagan R, Kelley W, Garcia-Gonzalo D . Whole-Genome Sequencing and Genetic Analysis Reveal Novel Stress Responses to Individual Constituents of Essential Oils in Escherichia coli. Appl Environ Microbiol. 2018; 84(7). PMC: 5861813. DOI: 10.1128/AEM.02538-17. View

20.

Ariza R, Cohen S, Bachhawat N, Levy S, Demple B . Repressor mutations in the marRAB operon that activate oxidative stress genes and multiple antibiotic resistance in Escherichia coli. J Bacteriol. 1994; 176(1):143-8. PMC: 205025. DOI: 10.1128/jb.176.1.143-148.1994. View