Allelic H3K27me3 to Allelic DNA Methylation Switch Maintains Noncanonical Imprinting in Extraembryonic Cells

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2020 Feb 18

PMID 32064321

Citations 58

Authors

Zhiyuan Chen

Qiangzong Yin

Azusa Inoue

Chunxia Zhang

Yi Zhang

Affiliations

Soon will be listed here.

Abstract

Faithful maintenance of genomic imprinting is essential for mammalian development. While germline DNA methylation-dependent (canonical) imprinting is relatively stable during development, the recently found oocyte-derived H3K27me3-mediated noncanonical imprinting is mostly transient in early embryos, with some genes important for placental development maintaining imprinted expression in the extraembryonic lineage. How these noncanonical imprinted genes maintain their extraembryonic-specific imprinting is unknown. Here, we report that maintenance of noncanonical imprinting requires maternal allele-specific de novo DNA methylation [i.e., somatic differentially methylated regions (DMRs)] at implantation. The somatic DMRs are located at the gene promoters, with paternal allele-specific H3K4me3 established during preimplantation development. Genetic manipulation revealed that both maternal EED and zygotic DNMT3A/3B are required for establishing somatic DMRs and maintaining noncanonical imprinting. Thus, our study not only reveals the mechanism underlying noncanonical imprinting maintenance but also sheds light on how histone modifications in oocytes may shape somatic DMRs in postimplantation embryos.

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