MiR-193a-5p Promotes Pancreatic Cancer Cell Metastasis Through SRSF6-mediated Alternative Splicing of OGDHL and ECM1

Overview

Journal Am J Cancer Res

Specialty Oncology

Date 2020 Feb 18

PMID 32064152

Citations 38

Authors

Manman Li

Pandi Wu

Zhaocong Yang

Siwei Deng

Lingyu Ni

Yanfeng Zhang

Liang Jin

Yi Pan

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) are short and non-coding RNAs binding to 3'UTR of target mRNAs to downregulate their expression. Recent studies have shown that miRNAs indirectly regulated alternative splicing (AS) by targeting splicing factors and caused shifts in splicing patterns of target genes. However, the roles of miRNA-regulating splicing factors in pancreatic cancer progression remain unknown. Herein, we reported that miR-193a-5p was markedly upregulated in pancreatic cancer tissues and cells and correlated with clinical outcomes of pancreatic cancer patients. Overexpression of miR-193a-5p contributed to the metastasis of pancreatic cancer cells both and . The mechanistic investigation suggested that miR-193a-5p modulated oxoglutarate dehydrogenase-like (OGDHL) and extracellular matrix protein 1 (ECM1) AS by targeting serine/arginine-rich splicing factor 6 (SRSF6), leading to the activation of the epithelial-to-mesenchymal transition (EMT) process. Together, our findings highlighted the role of miR-193a-5p-targeting SRSF6 in pancreatic cancer metastasis, which may serve as a novel target for pancreatic cancer diagnosis and therapy.

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