Antimicrobial Efficacy of 0.8% Hyaluronic Acid and 0.2% Chlorhexidine Against Strains: An In-vitro Study

Overview

Journal Pak J Med Sci

Specialty General Medicine

Date 2020 Feb 18

PMID 32063942

Citations 9

Authors

Munerah Binshabaib

Kawther Aabed

Fitoon Alotaibi

Milaf Alwaqid

Aljohara Alfraidy

Shatha Alharthi

Affiliations

Soon will be listed here.

Abstract

Objective: The aim of the present in-vitro study was to assess antimicrobial efficacy of 0.8% hyaluronic acid (HA) and 0.2% Chlorhexidine gluconate (CHX) against Porphyromonas gingivalis (P. gingivalis).

Methods: The study was performed between December 2018 and March 2019 at the College of Dentistry at the Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia. The P. gingivalis biofilms were formed and grown for 72 hours at 37°C under anaerobic conditions on glass slides coated with human saliva. The slides were individually positioned and exposed to 0.8% HA or 0.2% CHX. Therapeutically, the biofilms were divided into 3 groups as follows: (a) negative group; (b) 0.8% HA group and (c) 0.2% CHX group. P-values less than 0.05 were considered statistically significant.

Results: In the 0.8% HA group, P. gingivalis CFUs/ml were significantly higher at baseline than at 24- (P<0.05), 48 (P<0.05) and 72 hours (P<0.05) intervals. In the 0.2% CHX group, P. gingivalis CFUs/ml were significantly higher at baseline than at 72 hours interval (P<0.05). In the CHX group, there was no difference in P. gingivalis CFUs/ml between baseline, 24- and 48-hours intervals. At 48- and 72-hours intervals, the P. gingivalis CFUs/ml were significantly higher in the 0.2% CHX group compared with the 0.8% HA group.

Conclusion: In-vitro, 0.8% HA is more effective in reducing the P. gingivalis CFUs/ml compared with 0.2% CHX.

Citing Articles

Synthesis, characterization, and application of methylene blue functionalized reduced graphene oxide for photodynamic therapy in root canal treatment.

Jouhar R, Halim M, Ahmed M, Shah F, Quadri S Pak J Med Sci. 2025; 41(2):519-524.

PMID: 39926673 PMC: 11803785. DOI: 10.12669/pjms.41.2.11001.

Effectiveness of 0.2% Hyaluronic Acid on Clinical, Biomolecular and Microbiological Parameters in Type 2 Diabetes Mellitus Patients with Periodontitis.

Devina A, Halim F, Meivi M, Masulili S, Tadjoedin E, Lessang R Eur J Dent. 2024; 18(4):1090-1100.

PMID: 39074834 PMC: 11479742. DOI: 10.1055/s-0044-1782188.

The antimicrobial activity of tea tree oil () and its metal nanoparticles in oral bacteria.

Mohammed A, Aldahasi R, Rahman I, Shami A, Alotaibi M, BinShabaib M PeerJ. 2024; 12:e17241.

PMID: 38854801 PMC: 11162611. DOI: 10.7717/peerj.17241.

Hyaluronic acid-based gels for oral application: Comparison of effects on gingival cells and bacteria.

Marques A, Silva N, da Cruz M, Marques J, da Mata A J Oral Biol Craniofac Res. 2024; 14(3):238-244.

PMID: 38533132 PMC: 10963226. DOI: 10.1016/j.jobcr.2024.03.001.

The Effect of Adjunctive Use of Hyaluronic Acid on Prevalence of in Subgingival Biofilm in Patients with Chronic Periodontitis: A Systematic Review.

Alshehri F, Alharbi M Pharmaceutics. 2023; 15(7).

PMID: 37514069 PMC: 10385933. DOI: 10.3390/pharmaceutics15071883.

References

Eliezer M, Sculean A, Miron R, Nemcovsky C, Weinberg E, Weinreb M . Hyaluronic acid slows down collagen membrane degradation in uncontrolled diabetic rats. J Periodontal Res. 2019; 54(6):644-652. DOI: 10.1111/jre.12665. View

Javed F, Al Amri M, Al-Kheraif A, Qadri T, Ahmed A, Ghanem A . Efficacy of non-surgical periodontal therapy with adjunct Nd:YAG laser therapy in the treatment of periodontal inflammation among patients with and without type 2 diabetes mellitus: A short-term pilot study. J Photochem Photobiol B. 2015; 149:230-4. DOI: 10.1016/j.jphotobiol.2015.06.013. View

Bansal J, Kedige S, Anand S . Hyaluronic acid: a promising mediator for periodontal regeneration. Indian J Dent Res. 2010; 21(4):575-8. DOI: 10.4103/0970-9290.74232. View

Re A, Bonjovanni M, Ferreira M, Freitas O, Aires C . Effect of an Experimental Formulation Containing Chlorhexidine on Pathogenic Biofilms and Drug Release Behavior in the Presence or Absence of Bacteria. Pharmaceutics. 2019; 11(2). PMC: 6410057. DOI: 10.3390/pharmaceutics11020088. View

Suzuki N, Yoneda M, Hirofuji T . Mixed red-complex bacterial infection in periodontitis. Int J Dent. 2013; 2013:587279. PMC: 3606728. DOI: 10.1155/2013/587279. View

Toole B . Hyaluronan in morphogenesis. Semin Cell Dev Biol. 2001; 12(2):79-87. DOI: 10.1006/scdb.2000.0244. View

Chen M, Li L, Wang Z, Li P, Feng F, Zheng X . High molecular weight hyaluronic acid regulates P. gingivalis-induced inflammation and migration in human gingival fibroblasts via MAPK and NF-κB signaling pathway. Arch Oral Biol. 2018; 98:75-80. DOI: 10.1016/j.archoralbio.2018.10.027. View

Papaioannou W, Panagopoulos A, Koletsi-Kounari H, Kontou E, Makou M . Adhesion of Porphyromonas gingivalis and Biofilm Formation on Different Types of Orthodontic Brackets. Int J Dent. 2012; 2012:471380. PMC: 3272364. DOI: 10.1155/2012/471380. View

Vanden Bogaerde L . Treatment of infrabony periodontal defects with esterified hyaluronic acid: clinical report of 19 consecutive lesions. Int J Periodontics Restorative Dent. 2009; 29(3):315-23. View

10.

Kaur A, Bhavikatti S, Das S, Khanna S, Jain M, Kaur A . Efficacy of Ozonised Water and 0.2% Chlorhexidine Gluconate in the Management of Chronic Periodontitis when Used as an Irrigant in Conjugation with Phase I Therapy. J Contemp Dent Pract. 2019; 20(3):318-323. View

11.

Neuman M, Nanau R, Oruna-Sanchez L, Coto G . Hyaluronic acid and wound healing. J Pharm Pharm Sci. 2015; 18(1):53-60. DOI: 10.18433/j3k89d. View

12.

Javed F, Nasstrom K, Benchimol D, Altamash M, Klinge B, Engstrom P . Comparison of periodontal and socioeconomic status between subjects with type 2 diabetes mellitus and non-diabetic controls. J Periodontol. 2007; 78(11):2112-9. DOI: 10.1902/jop.2007.070186. View

13.

Abduljabbar T, Vohra F, Javed F, Akram Z . Antimicrobial photodynamic therapy adjuvant to non-surgical periodontal therapy in patients with diabetes mellitus: A meta-analysis. Photodiagnosis Photodyn Ther. 2016; 17:138-146. DOI: 10.1016/j.pdpdt.2016.11.008. View

14.

Vianna M, Gomes B, Berber V, Zaia A, Ferraz C, de Souza-Filho F . In vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004; 97(1):79-84. DOI: 10.1016/s1079-2104(03)00360-3. View

15.

Al-Shammari N, Shafshak S, Ali M . Effect of 0.8% Hyaluronic Acid in Conventional Treatment of Moderate to Severe Chronic Periodontitis. J Contemp Dent Pract. 2018; 19(5):527-534. View

16.

Kruck C, Eick S, Knofler G, Purschwitz R, Jentsch H . Clinical and microbiologic results 12 months after scaling and root planing with different irrigation solutions in patients with moderate chronic periodontitis: a pilot randomized trial. J Periodontol. 2011; 83(3):312-20. DOI: 10.1902/jop.2011.110044. View

17.

Sukumar S, Drizhal I . Hyaluronic acid and periodontitis. Acta Medica (Hradec Kralove). 2008; 50(4):225-8. View

18.

Xie Z, Meng K, Yang X, Liu J, Yu J, Zheng C . Identification of a Quorum Sensing System Regulating Capsule Polysaccharide Production and Biofilm Formation in . Front Cell Infect Microbiol. 2019; 9:121. PMC: 6482233. DOI: 10.3389/fcimb.2019.00121. View

19.

Javed F, Ahmed H, Mehmood A, Bain C, Romanos G . Effect of nonsurgical periodontal therapy (with or without oral doxycycline delivery) on glycemic status and clinical periodontal parameters in patients with prediabetes: a short-term longitudinal randomized case-control study. Clin Oral Investig. 2014; 18(8):1963-8. DOI: 10.1007/s00784-014-1185-6. View

20.

Javed F, Al-Askar M, Al-Rasheed A, Babay N, Galindo-Moreno P, Al-Hezaimi K . Comparison of self-perceived oral health, periodontal inflammatory conditions and socioeconomic status in individuals with and without prediabetes. Am J Med Sci. 2011; 344(2):100-4. DOI: 10.1097/MAJ.0b013e31823650a7. View