Effect of Focal Adhesion Kinase Inhibition on Osteoblastic Cells Grown on Titanium with Different Topographies

Overview

Journal J Appl Oral Sci

Specialty Dentistry

Date 2020 Feb 13

PMID 32049134

Citations 10

Authors

Helena Bacha Lopes

Alann Thaffarell Portilho Souza

Gileade Pereira Freitas

Carlos Nelson Elias

Adalberto Luiz Rosa

Marcio Mateus Beloti

Affiliations

Soon will be listed here.

Abstract

Objective: The present study aimed to investigate the participation of focal adhesion kinases (FAK) in interactions between osteoblastic cells and titanium (Ti) surfaces with three different topographies, namely, untreated (US), microstructured (MS), and nanostructured (NS).

Methodology: Osteoblasts harvested from the calvarial bones of 3-day-old rats were cultured on US, MS and NS discs in the presence of PF-573228 (FAK inhibitor) to evaluate osteoblastic differentiation. After 24 h, we evaluated osteoblast morphology and vinculin expression, and on day 10, the following parameters: gene expression of osteoblastic markers and integrin signaling components, FAK protein expression and alkaline phosphatase (ALP) activity. A smooth surface, porosities at the microscale level, and nanocavities were observed in US, MS, and NS, respectively.

Results: FAK inhibition decreased the number of filopodia in cells grown on US and MS compared with that in NS. FAK inhibition decreased the gene expression of Alp, bone sialoprotein, osteocalcin, and ALP activity in cells grown on all evaluated surfaces. FAK inhibition did not affect the gene expression of Fak, integrin alpha 1 ( Itga1 ) and integrin beta 1 ( Itgb1 ) in cells grown on MS, increased the gene expression of Fak in cells grown on NS, and increased the gene expression of Itga1 and Itgb1 in cells grown on US and NS. Moreover, FAK protein expression decreased in cells cultured on US but increased in cells cultured on MS and NS after FAK inhibition; no difference in the expression of vinculin was observed among cells grown on all surfaces.

Conclusions: Our data demonstrate the relevance of FAK in the interactions between osteoblastic cells and Ti surfaces regardless of surface topography. Nanotopography positively regulated FAK expression and integrin signaling pathway components during osteoblast differentiation. In this context, the development of Ti surfaces with the ability to upregulate FAK activity could positively impact the process of implant osseointegration.

Citing Articles

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PMID: 39057858 PMC: 11279432. DOI: 10.3390/nano14141181.

A New α + β Ti-15Nb Alloy with Low Elastic Modulus: Characterization and In Vitro Evaluation on Osteogenic Phenotype.

Donato T, Dos Santos Jorge Sousa K, Kuroda P, Grandini C J Funct Biomater. 2023; 14(9).

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Effects of Modulation of the Hedgehog and Notch Signaling Pathways on Osteoblast Differentiation Induced by Titanium with Nanotopography.

Souza P, Adolpho L, Lopes H, Weffort D, Souza A, Oliveira F J Funct Biomater. 2023; 14(2).

PMID: 36826878 PMC: 9968096. DOI: 10.3390/jfb14020079.

Influence of the roughness of dental implants obtained by additive manufacturing on osteoblastic adhesion and proliferation: A systematic review.

Tardelli J, Duarte Firmino A, Ferreira I, Dos Reis A Heliyon. 2023; 8(12):e12505.

PMID: 36643331 PMC: 9834751. DOI: 10.1016/j.heliyon.2022.e12505.

Topography-mediated immunomodulation in osseointegration; Ally or Enemy.

Shirazi S, Ravindran S, Cooper L Biomaterials. 2022; 291:121903.

PMID: 36410109 PMC: 10148651. DOI: 10.1016/j.biomaterials.2022.121903.

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