MicroRNA-497-5p Functions As a Modulator of Apoptosis by Regulating Metadherin in Ovarian Cancer

Overview

Journal Cell Transplant

Specialties Cell Biology
General Surgery

Date 2020 Feb 13

PMID 32046519

Citations 8

Authors

Chunyan Liu

Anne Bordeaux

Stanka Hettich

Suhui Han

Affiliations

Soon will be listed here.

Abstract

Ovarian cancer (OC) has a high mortality rate among women worldwide. However, even with the advances in detection and therapeutics, the number of cases is increasing worldwide. Increasingly, microRNAs (miRNAs), including miR-497-5p, have been implicated in the progression of many cancers, but the role of miR-497-5p in OC remains unknown. The purpose of this study was to investigate the underlying molecular mechanism of miR-497-5p in OC. Herein, we find that miR-497-5p is down-regulated in OC tissues, and overexpression of miR-497-5p enhances apoptosis in OC cells. The increased apoptosis was correlated with enhanced expression of apoptosis-related proteins. MiR-497-5p directly bound the 3'-untranslated region of metadherin (MTDH), leading to the reduction of MTDH in mRNA and protein levels. Moreover, MTDH knockout promoted the apoptosis of OC cells. Taken together, we conclude that miR-497-5p contributes to cell apoptosis in OC by regulating MTDH.

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References

Sun Z, Li A, Yu Z, Li X, Guo X, Chen R . MicroRNA-497-5p Suppresses Tumor Cell Growth of Osteosarcoma by Targeting ADP Ribosylation Factor-Like Protein 2. Cancer Biother Radiopharm. 2017; 32(10):371-378. DOI: 10.1089/cbr.2017.2268. View

Chen J, Jia Y, Jia Z, Zhu Y, Jin Y . Silencing the expression of MTDH increases the radiation sensitivity of SKOV3 ovarian cancer cells and reduces their proliferation and metastasis. Int J Oncol. 2018; 53(5):2180-2190. DOI: 10.3892/ijo.2018.4541. View

Yan J, Zhang Y, Liao J, Ke K, Chang Y, Li P . MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1. Oncotarget. 2015; 6(30):29527-42. PMC: 4745744. DOI: 10.18632/oncotarget.5012. View

Mansoori B, Mohammadi A, Ghasabi M, Shirjang S, Dehghan R, Montazeri V . miR-142-3p as tumor suppressor miRNA in the regulation of tumorigenicity, invasion and migration of human breast cancer by targeting Bach-1 expression. J Cell Physiol. 2018; 234(6):9816-9825. DOI: 10.1002/jcp.27670. View

Chen X, Li X, Long M, Wang X, Gao Z, Cui Y . The FBXW7 tumor suppressor inhibits breast cancer proliferation and promotes apoptosis by targeting MTDH for degradation. Neoplasma. 2018; 65(2):201-209. DOI: 10.4149/neo_2018_170228N149. View

Gao Y, Wu J . MicroRNA-200c and microRNA-141 as potential diagnostic and prognostic biomarkers for ovarian cancer. Tumour Biol. 2015; 36(6):4843-50. DOI: 10.1007/s13277-015-3138-3. View

Huang X, Wang L, Liu W, Li F . MicroRNA-497-5p inhibits proliferation and invasion of non-small cell lung cancer by regulating FGF2. Oncol Lett. 2019; 17(3):3425-3431. PMC: 6396182. DOI: 10.3892/ol.2019.9954. View

Croset M, Pantano F, Kan C, Bonnelye E, Descotes F, Alix-Panabieres C . miRNA-30 Family Members Inhibit Breast Cancer Invasion, Osteomimicry, and Bone Destruction by Directly Targeting Multiple Bone Metastasis-Associated Genes. Cancer Res. 2018; 78(18):5259-5273. DOI: 10.1158/0008-5472.CAN-17-3058. View

Chen Y, Kuang D, Zhao X, Chen D, Wang X, Yang Q . miR-497-5p inhibits cell proliferation and invasion by targeting KCa3.1 in angiosarcoma. Oncotarget. 2016; 7(36):58148-58161. PMC: 5295420. DOI: 10.18632/oncotarget.11252. View

10.

Ran F, Hsu P, Wright J, Agarwala V, Scott D, Zhang F . Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013; 8(11):2281-2308. PMC: 3969860. DOI: 10.1038/nprot.2013.143. View

11.

Siegel R, Miller K, Jemal A . Cancer Statistics, 2017. CA Cancer J Clin. 2017; 67(1):7-30. DOI: 10.3322/caac.21387. View

12.

Qiu Y, Yu H, Shi X, Xu K, Tang Q, Liang B . microRNA-497 inhibits invasion and metastasis of colorectal cancer cells by targeting vascular endothelial growth factor-A. Cell Prolif. 2016; 49(1):69-78. PMC: 6495881. DOI: 10.1111/cpr.12237. View

13.

Li G, Wang K, Wang J, Qin S, Sun X, Ren H . miR-497-5p inhibits tumor cell growth and invasion by targeting SOX5 in non-small-cell lung cancer. J Cell Biochem. 2019; 120(6):10587-10595. DOI: 10.1002/jcb.28345. View

14.

Zhang L, Singh A, Plaisier C, Pruett N, Ripley R, Schrump D . Metadherin Is a Prognostic Apoptosis Modulator in Mesothelioma Induced via NF-κB-Mediated Signaling. Transl Oncol. 2019; 12(6):859-870. PMC: 6500914. DOI: 10.1016/j.tranon.2019.03.005. View

15.

Tian W, Hao S, Gao B, Jiang Y, Zhang X, Zhang S . Lobaplatin inhibits breast cancer progression, cell proliferation while it induces cell apoptosis by downregulating MTDH expression. Drug Des Devel Ther. 2018; 12:3563-3571. PMC: 6211578. DOI: 10.2147/DDDT.S163157. View

16.

Dwivedi S, Mustafi S, Mangala L, Jiang D, Pradeep S, Rodriguez-Aguayo C . Therapeutic evaluation of microRNA-15a and microRNA-16 in ovarian cancer. Oncotarget. 2016; 7(12):15093-104. PMC: 4924772. DOI: 10.18632/oncotarget.7618. View

17.

Zhao C, Huang C, Weng T, Xiao X, Ma H, Liu L . Computational prediction of MicroRNAs targeting GABA receptors and experimental verification of miR-181, miR-216 and miR-203 targets in GABA-A receptor. BMC Res Notes. 2012; 5:91. PMC: 3296612. DOI: 10.1186/1756-0500-5-91. View

18.

Choi Y, Meghani K, Brault M, Leclerc L, He Y, Day T . Platinum and PARP Inhibitor Resistance Due to Overexpression of MicroRNA-622 in BRCA1-Mutant Ovarian Cancer. Cell Rep. 2016; 14(3):429-439. PMC: 4731274. DOI: 10.1016/j.celrep.2015.12.046. View

19.

Yang L, Tian Y, Leong W, Song H, Yang W, Wang M . Efficient and tumor-specific knockdown of MTDH gene attenuates paclitaxel resistance of breast cancer cells both in vivo and in vitro. Breast Cancer Res. 2018; 20(1):113. PMC: 6145322. DOI: 10.1186/s13058-018-1042-7. View

20.

Zhao H, Liu S, Wang G, Wu X, Ding Y, Guo G . Expression of miR-136 is associated with the primary cisplatin resistance of human epithelial ovarian cancer. Oncol Rep. 2014; 33(2):591-8. DOI: 10.3892/or.2014.3640. View