Micronucleus Test of DHU001, a Polyherbal Formula, in Bone Marrow Cells of Male ICR Mice

Overview

Journal Toxicol Res

Specialty Toxicology

Date 2020 Feb 11

PMID 32038842

Citations 2

Authors

Seong-Soo Roh

Hyeung-Sik Lee

Sae-Kwang Ku

Affiliations

Soon will be listed here.

Abstract

The genotoxic effects of DHU001, a polyherbal formula were evaluated using the mouse micronucleus test. DHU001 was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2000, 1000 and 500 mg/kg. Cyclophosphamide was used as a known geno-toxic agent in a positive control. The appearance of a micronucleus is used as an index for genotoxic potential. In addition, the changes on the total white blood cells and differential counts on the lymphocytes, neutrophils, eosinophils, basophils and monocytes in the prepared blood smears were also conducted to observe the possible immunosuppression. The results indicats that DHU001 showed no genotoxicity effects up to 2000 mg/kg dosing levels and did not influenced on the total white blood cells and differential counts. In addition, it is also considered that there were no problems from cytotoxicity of DHU001 tested in this study because the polychromatic erythrocyte ratio was detected as > 0.41 in all tested groups.

Citing Articles

Micronucleus Test of Picrorrhiza Rhizoma Aqueous Extract in Bone Marrow Cells of Male ICR Mice.

Chung I, Cheon W, Ku S Toxicol Res. 2013; 27(2):119-23.

PMID: 24278560 PMC: 3834366. DOI: 10.5487/TR.2011.27.2.119.

Effect of DHU001, a Polyherbal Formula on Formalin-induced Paw Chronic Inflammation of Mice.

Cho Y, Chung I, Cheon W, Lee H, Ku S Toxicol Res. 2013; 27(2):95-102.

PMID: 24278557 PMC: 3834374. DOI: 10.5487/TR.2011.27.2.095.

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