A Possible Role of the Aleurone Expressed Gene in the Hydrolysis of the Cell Wall Mannans of the Starchy Endosperm in Germinating L. Seeds

Overview

Journal Front Plant Sci

Date 2020 Feb 11

PMID 32038680

Citations 6

Authors

Raquel Iglesias-Fernandez

Elena Pastor-Mora

Jesus Vicente-Carbajosa

Pilar Carbonero

Affiliations

Soon will be listed here.

Abstract

The barley endo-β-mannanase () gene family () has been identified and the expression of its members analyzed throughout different plant organs, and upon grain development and germination. The gene has been found to be highly expressed in developing and germinating grains. The MAN (EC 3.2.1.78) enzymatic activity gets a maximum in grains at 48 h of germination (post-germination event). Immunolocalization of mannan polymers in grains has revealed the presence of these polysaccharides in the endosperm cell walls (CWs). By mRNA hybridization assays, the transcripts have been localized to the aleurone layer, but not to the dead starchy endosperm cells. These data suggest that MAN1 is synthesized in the aleurone layer during early grain imbibition and moves potentially through the apoplast to the endosperm where the hydrolysis of the mannan polymers takes place after germination . Hence, mannans in the starchy endosperm CWs, besides their structural function, could be used as reserve compounds upon barley post-germination.

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