Blastulation of a Zygote to a Hatched Blastocyst Without Any Clear Cell Division: an Observational Finding in a Time-lapse System After in Vitro Fertilization

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2020 Feb 7

PMID 32026203

Authors

N L Sandi-Monroy

S Musanovic

D Zhu

K Eibner

N Reeka

J Koglin

K Bundschu

F Gagsteiger

Affiliations

Soon will be listed here.

Abstract

Purpose: To describe an interesting not previously described morphokinetic finding.

Methods: Retrospective case report of a couple undergoing controlled ovarian stimulation (COS) followed by in vitro fertilization and blastocyst transfer.

Results: We identified a unique finding of blastulation of a fertilized human zygote after conventional in vitro fertilization. The fertilized zygote did not show any clear cytokinesis until approximately 107 h post insemination, when it started dividing into a blastocyst. By 113 h post insemination, inner cell mass and trophectoderm cells could be clearly distinguished and the blastocyst was completely hatched by 136 h post insemination.

Conclusion: Time-lapse systems offer more detailed observations of embryonic development. Here, we report an atypical development of an embryo that was not described previously. We hope to become an insightful discussion among peers and incentive the publication of such findings in the future.

References

Fujiwara T, Bandi M, Nitta M, Ivanova E, Bronson R, Pellman D . Cytokinesis failure generating tetraploids promotes tumorigenesis in p53-null cells. Nature. 2005; 437(7061):1043-7. DOI: 10.1038/nature04217. View

Li M, Ma S, Yang H, Wu K, Zhong W, Yu G . Pregnancy with oocytes characterized by narrow perivitelline space and heterogeneous zona pellucida: is intracytoplasmic sperm injection necessary?. J Assist Reprod Genet. 2014; 31(3):285-94. PMC: 3947078. DOI: 10.1007/s10815-013-0169-9. View

Gardner D, Meseguer M, Rubio C, Treff N . Diagnosis of human preimplantation embryo viability. Hum Reprod Update. 2015; 21(6):727-47. DOI: 10.1093/humupd/dmu064. View

Margall-Ducos G, Celton-Morizur S, Couton D, Bregerie O, Desdouets C . Liver tetraploidization is controlled by a new process of incomplete cytokinesis. J Cell Sci. 2007; 120(Pt 20):3633-9. DOI: 10.1242/jcs.016907. View

Campbell A, Fishel S, Bowman N, Duffy S, Sedler M, Hickman C . Modelling a risk classification of aneuploidy in human embryos using non-invasive morphokinetics. Reprod Biomed Online. 2013; 26(5):477-85. DOI: 10.1016/j.rbmo.2013.02.006. View

Zanetti B, Braga D, Setti A, Figueira R, Iaconelli Jr A, Borges Jr E . Is perivitelline space morphology of the oocyte associated with pregnancy outcome in intracytoplasmic sperm injection cycles?. Eur J Obstet Gynecol Reprod Biol. 2018; 231:225-229. DOI: 10.1016/j.ejogrb.2018.10.053. View

Desai N, Ploskonka S, Goodman L, Austin C, Goldberg J, Falcone T . Analysis of embryo morphokinetics, multinucleation and cleavage anomalies using continuous time-lapse monitoring in blastocyst transfer cycles. Reprod Biol Endocrinol. 2014; 12:54. PMC: 4074839. DOI: 10.1186/1477-7827-12-54. View

Sciorio R, Thong K, Pickering S . Spontaneous blastocyst collapse as an embryo marker of low pregnancy outcome: A Time-Lapse study. JBRA Assist Reprod. 2019; 24(1):34-40. PMC: 6993169. DOI: 10.5935/1518-0557.20190044. View

Coticchio G, Mignini Renzini M, Novara P, Lain M, De Ponti E, Turchi D . Focused time-lapse analysis reveals novel aspects of human fertilization and suggests new parameters of embryo viability. Hum Reprod. 2017; 33(1):23-31. DOI: 10.1093/humrep/dex344. View

10.

Sauerbrun-Cutler M, Vega M, Breborowicz A, Gonzales E, Stein D, Lederman M . Oocyte zona pellucida dysmorphology is associated with diminished in-vitro fertilization success. J Ovarian Res. 2015; 8:5. PMC: 4355133. DOI: 10.1186/s13048-014-0111-5. View

11.

Schubiger G . Adult differentiation from partial Drosophila embryos after egg ligation during stages of nuclear multiplication and cellular blastoderm. Dev Biol. 1976; 50(2):476-88. DOI: 10.1016/0012-1606(76)90166-4. View

12.

Vignery A . Osteoclasts and giant cells: macrophage-macrophage fusion mechanism. Int J Exp Pathol. 2001; 81(5):291-304. PMC: 2517739. DOI: 10.1111/j.1365-2613.2000.00164.x. View

13.

Deng S, Azevedo M, Baylies M . Acting on identity: Myoblast fusion and the formation of the syncytial muscle fiber. Semin Cell Dev Biol. 2017; 72:45-55. PMC: 5910025. DOI: 10.1016/j.semcdb.2017.10.033. View

14.

Chen E, Grote E, Mohler W, Vignery A . Cell-cell fusion. FEBS Lett. 2007; 581(11):2181-93. DOI: 10.1016/j.febslet.2007.03.033. View

15.

Frescas D, Mavrakis M, Lorenz H, Delotto R, Lippincott-Schwartz J . The secretory membrane system in the Drosophila syncytial blastoderm embryo exists as functionally compartmentalized units around individual nuclei. J Cell Biol. 2006; 173(2):219-30. PMC: 2063813. DOI: 10.1083/jcb.200601156. View

16.

Sousa M, Teixeira da Silva J, Silva J, Cunha M, Viana P, Oliveira E . Embryological, clinical and ultrastructural study of human oocytes presenting indented zona pellucida. Zygote. 2013; 23(1):145-57. DOI: 10.1017/S0967199413000403. View

17.

Meriano J, Clark C, Cadesky K, Laskin C . Binucleated and micronucleated blastomeres in embryos derived from human assisted reproduction cycles. Reprod Biomed Online. 2004; 9(5):511-20. DOI: 10.1016/s1472-6483(10)61635-5. View

18.

Wong C, Loewke K, Bossert N, Behr B, De Jonge C, Baer T . Non-invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage. Nat Biotechnol. 2010; 28(10):1115-21. DOI: 10.1038/nbt.1686. View

19.

Loncar D, Singer S . Cell membrane formation during the cellularization of the syncytial blastoderm of Drosophila. Proc Natl Acad Sci U S A. 1995; 92(6):2199-203. PMC: 42451. DOI: 10.1073/pnas.92.6.2199. View

20.

Salas-Vidal E, Lomeli H . Imaging filopodia dynamics in the mouse blastocyst. Dev Biol. 2003; 265(1):75-89. DOI: 10.1016/j.ydbio.2003.09.012. View