A Visual FRET Immunofluorescent Biosensor for Ratiometric Parathyroid Hormone (1-84) Antigen Point-of-Care Detection

Overview

Journal J Fluoresc

Specialties Biophysics
Chemistry

Date 2020 Feb 6

PMID 32020436

Authors

Yixin Nie

Jingting Li

Yang Liu

Qian Zhang

Qiang Ma

Affiliations

Soon will be listed here.

Abstract

Excessive secretion of PTH leads to disturbance of calcium and phosphorus metabolism in the body, which promotes bone, kidney, digestive system and nervous system diseases. Due to the short half-life of PTH, it becomes a difficult issue for PTH detection in the clinical diagnosis field. We explored a competitive immunofluorescent sensing mode based on FRET of two-color CdTe QDs for ratiometric PTH 1-84 antigen detection. The FRET effect and ratiometric fluorescence between the two-color CdTe QDs motivated accurate quantification of PTH 1-84 antigen concentration from 0.01 ng mL to 0.08 ng mL with a limit of detection of 3 pg mL. More importantly, under UV irradiation, samples with different concentrations of PTH 1-84 antigen achieved fluorescence visualization, which provides huge possibility for the practical application of PTH 1-84 antigen point-of-care detection.

References

Escosura-Muniz A, Espinoza-Castaneda M, Chamorro-Garcia A, Rodriguez-Hernandez C, de Torres C, Merkoci A . In situ monitoring of PTHLH secretion in neuroblastoma cells cultured onto nanoporous membranes. Biosens Bioelectron. 2018; 107:62-68. DOI: 10.1016/j.bios.2018.01.064. View

Mehta A, Im J, Kim B, Min H, Nie R, Seok S . Stabilization of Lead-Tin-Alloyed Inorganic-Organic Halide Perovskite Quantum Dots. ACS Nano. 2018; 12(12):12129-12139. DOI: 10.1021/acsnano.8b05478. View

Maldaner F, Perobelli R, Xavier B, Remuzzi G, Walter M, Dalmora S . Evaluation of recombinant human parathyroid hormone by CZE method and its correlation with in vitro bioassay and LC methods. Talanta. 2016; 162:567-573. DOI: 10.1016/j.talanta.2016.10.053. View

Li S, Liu Y, Ma Q . A novel polydopamine electrochemiluminescence organic nanoparticle-based biosensor for parathyroid hormone detection. Talanta. 2019; 202:540-545. DOI: 10.1016/j.talanta.2019.05.022. View

Yu X, Wen K, Wang Z, Zhang X, Li C, Zhang S . General Bioluminescence Resonance Energy Transfer Homogeneous Immunoassay for Small Molecules Based on Quantum Dots. Anal Chem. 2016; 88(7):3512-20. DOI: 10.1021/acs.analchem.5b03581. View

Otieno B, Krause C, Jones A, Kremer R, Rusling J . Cancer Diagnostics via Ultrasensitive Multiplexed Detection of Parathyroid Hormone-Related Peptides with a Microfluidic Immunoarray. Anal Chem. 2016; 88(18):9269-75. PMC: 5032051. DOI: 10.1021/acs.analchem.6b02637. View

Urdea M, Penny L, Olmsted S, Giovanni M, Kaspar P, Shepherd A . Requirements for high impact diagnostics in the developing world. Nature. 2006; 444 Suppl 1:73-9. DOI: 10.1038/nature05448. View

Kotresh M, Adarsh K, Shivkumar M, Mulimani B, Savadatti M, Inamdar S . Spectroscopic investigation of alloyed quantum dot-based FRET to cresyl violet dye. Luminescence. 2015; 31(3):760-8. DOI: 10.1002/bio.3021. View

Liu J, Geng Z, Fan Z, Liu J, Chen H . Point-of-care testing based on smartphone: The current state-of-the-art (2017-2018). Biosens Bioelectron. 2019; 132:17-37. DOI: 10.1016/j.bios.2019.01.068. View

10.

Crivianu-Gaita V, Aamer M, Posaratnanathan R, Romaschin A, Thompson M . Acoustic wave biosensor for the detection of the breast and prostate cancer metastasis biomarker protein PTHrP. Biosens Bioelectron. 2015; 78:92-99. DOI: 10.1016/j.bios.2015.11.031. View

11.

Espinoza-Castaneda M, Escosura-Muniz A, Chamorro A, de Torres C, Merkoci A . Nanochannel array device operating through Prussian blue nanoparticles for sensitive label-free immunodetection of a cancer biomarker. Biosens Bioelectron. 2014; 67:107-14. DOI: 10.1016/j.bios.2014.07.039. View

12.

Chan H, Tan M, Wu H . Point-of-care testing: applications of 3D printing. Lab Chip. 2017; 17(16):2713-2739. DOI: 10.1039/c7lc00397h. View

13.

Ozcan H, Yildiz K, Cakar C, Aydin T, Asav E, Sagiroglu A . Ultrasensitive Impedimetric Biosensor Fabricated by a New Immobilisation Technique for Parathyroid Hormone. Appl Biochem Biotechnol. 2015; 176(5):1251-62. DOI: 10.1007/s12010-015-1643-x. View

14.

Sayikli Simsek C, Sonuc Karaboga M, Sezginturk M . A new immobilization procedure for development of an electrochemical immunosensor for parathyroid hormone detection based on gold electrodes modified with 6-mercaptohexanol and silane. Talanta. 2015; 144:210-8. DOI: 10.1016/j.talanta.2015.06.010. View