Transgenic Mice Overexpressing Human and in Neurons Exhibit Changes in Behavior and Calcium Homeostasis but Show No Signs of Neurodegeneration

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 Feb 5

PMID 32012922

Citations 4

Authors

Lukasz Majewski

Filip Maciag

Pawel M Boguszewski

Jacek Kuznicki

Affiliations

Soon will be listed here.

Abstract

The maintenance of proper cytosolic Ca level is crucial for neuronal survival, and dysregulation of Ca homeostasis is found in a variety of neurological disorders, including Alzheimer's disease. According to the "Ca hypothesis of aging", Ca disturbances precede the onset of AD symptoms and lead to neurodegeneration. STIM and ORAI proteins are involved in neuronal physiological and pathological processes as essential components of the store-operated Ca entry. Our previous data suggested that overexpression of and might increase basal neuronal cytosolic Ca level. We generated double transgenic mice overexpressing these two genes in neurons, expecting that the increased basal Ca concentration will lead to premature neurodegeneration. We observed changes in Ca homeostasis and electrophysiological properties in acute brain slices of STIM2/ORAI1 neurons. However, we did not observe any augmentation of neurodegenerative processes, as tested by Fluoro-Jade C staining and assessment of amyloidogenesis. The battery of behavioral tests did not show any signs of accelerated aging. We conclude that changes of calcium homeostasis induced by overexpression of and had no substantial adverse effects on neurons and did not lead to early neurodegeneration.

Citing Articles

STIM Proteins: The Gas and Brake of Calcium Entry in Neurons.

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