High-Quality Genome Assembly of Reveals Its Unique Genome Evolution

Overview

Journal Front Genet

Date 2020 Feb 4

PMID 32010195

Citations 11

Authors

Boping Tang

Zhongkai Wang

Qiuning Liu

Huabin Zhang

Senhao Jiang

XinZheng Li

Zhengfei Wang

Yue Sun

Zhongli Sha

Hui Jiang

Xugan Wu

Yandong Ren

Haorong Li

Fujun Xuan

Baoming Ge

Wei Jiang

Shusheng She

Hongying Sun

Qiang Qiu

Wen Wang

Qun Wang

Gaofeng Qiu

Daizhen Zhang

Yongxin Li

Affiliations

Soon will be listed here.

Abstract

As an important freshwater aquaculture species in China, the Chinese mitten crab () has high economic and nutritional value. However, limited genomic information is currently available for studying its basic development and genetic diversity. Here, we performed whole-genome sequencing on Oxford Nanopore Technologies Limited's platform using promethION. The assembled size of genome was approximately 1.27 Gb, which is close to the estimated size (1.19 Gb). Furthermore, based on assessment using Benchmarking Universal Single-Copy Orthologs (BUSCO) (Simao et al., 2015), 94.00% of the expected eukaryotic genes were completely present in the genome assembly. In addition, repetitive sequences accounted for ~61.42% of the assembled genome, and 22,619 protein-coding genes were annotated. Comparative genomics analysis demonstrated that the Chinese mitten crab diverged from ~373.6 million years ago, with a faster evolution rate than shrimp. We anticipate that the annotated high-quality genome of will promote research on its basic development and evolution and make substantial contributions to comparative genomic analyses of crustaceans.

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