Long Noncoding RNA SNHG17 Induced by YY1 Facilitates the Glioma Progression Through Targeting MiR-506-3p/CTNNB1 Axis to Activate Wnt/β-catenin Signaling Pathway

Overview

Journal Cancer Cell Int

Publisher Biomed Central

Date 2020 Feb 4

PMID 32009853

Citations 24

Authors

Huixia Li

Tianhao Li

Dehai Huang

Peng Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Glioma is one of the most widely diagnosed malignancies worldwide. It has been reported that long noncoding RNAs (lncRNAs) are participators in the tumorgenesis of cancers. Nevertheless, the role and function of lncRNA SNHG17 among glioma is unclear.

Methods: RT-qPCR revealed SNHG17, YY1, miR-506-3p, CTNNB1 expression among glioma cells. CCK-8, colony formation, EdU, flow cytometry, TUNEL and western blot assays revealed the function of SNHG17 in glioma. RIP uncovered SNHG17, miR-506-3p and CTNNB1 enrichment in RISC complex. Luciferase reporter assays and RNA pull down revealed interaction of miR-506-3p with SNHG17 and CTNNB1.

Results: SNHG17 expression was up-regulated in glioma tissues and cells. SNHG17 silence attenuated cell proliferation and promoted apoptosis and repressed tumor growth. Moreover, SNHG17 was up-regulated by transcription factor YY1. Mechanistically, SNHG17 activated Wnt/β-catenin signaling pathway in glioma. CTNNB1 was referred to as the mRNA of β-catenin, we validated that SNHG17 bound to miR-506-3p to induce CTNNB1 and activate Wnt/β-catenin signaling pathway. Rescue experiments indicated that CTNNB1 overexpression abolished the inhibitory effects of SNHG7 inhibition on glioma progression.

Conclusions: The findings that YY1-induced SNHG17 facilitated the glioma progression through targeting miR-506-3p/CTNNB1 axis to activate Wnt/β-catenin signaling pathway offered a brand-new prospects to molecular-targeted treatment for glioma.

Citing Articles

Yin Yang 1: Function, Mechanisms, and Glia.

Rodriguez-Campuzano A, Castelan F, Hernandez-Kelly L, Felder-Schmittbuhl M, Ortega A Neurochem Res. 2025; 50(2):96.

PMID: 39904836 PMC: 11794380. DOI: 10.1007/s11064-025-04345-7.

New sights on long non-coding RNAs in glioblastoma: A review of molecular mechanism.

Bagheri-Mohammadi S, Karamivandishi A, Mahdavi S, Siahposht-Khachaki A Heliyon. 2024; 10(21):e39744.

PMID: 39553554 PMC: 11564028. DOI: 10.1016/j.heliyon.2024.e39744.

YY1-induced lncRNA00511 promotes melanoma progression via the miR-150-5p/ADAM19 axis.

Chen Y, Fu X, Guo H, Fu X, Shi K, Gao T Am J Cancer Res. 2024; 14(2):809-831.

PMID: 38455406 PMC: 10915319.

Interplay between lncRNA/miRNA and Wnt/ß-catenin signaling in brain cancer tumorigenesis.

Rahmani F, Al-Asady A, Hanaie R, Zandigohar M, Faridnejad H, Payazdan M EXCLI J. 2024; 22:1211-1222.

PMID: 38204968 PMC: 10776877. DOI: 10.17179/excli2023-6490.

YY1 Knockdown Relieves the Differentiation Block and Restores Apoptosis in AML Cells.

Noguera N, Travaglini S, Scalea S, Catalanotto C, Reale A, Zampieri M Cancers (Basel). 2023; 15(15).

PMID: 37568827 PMC: 10417667. DOI: 10.3390/cancers15154010.

References

Ranzani V, Rossetti G, Panzeri I, Arrigoni A, Bonnal R, Curti S . The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4. Nat Immunol. 2015; 16(3):318-325. PMC: 4333215. DOI: 10.1038/ni.3093. View

Yang S, Lee K, Lee J, Lee J, Lee K, Rhee J . Inhibition of SCAMP1 suppresses cell migration and invasion in human pancreatic and gallbladder cancer cells. Tumour Biol. 2013; 34(5):2731-9. DOI: 10.1007/s13277-013-0825-9. View

Xu L, Fu H, Zhang J, Feng F, Wang Q, Zhu X . Impaired Function of Bone Marrow Mesenchymal Stem Cells from Immune Thrombocytopenia Patients in Inducing Regulatory Dendritic Cell Differentiation Through the Notch-1/Jagged-1 Signaling Pathway. Stem Cells Dev. 2017; 26(22):1648-1661. DOI: 10.1089/scd.2017.0078. View

Ricard D, Idbaih A, Ducray F, Lahutte M, Hoang-Xuan K, Delattre J . Primary brain tumours in adults. Lancet. 2012; 379(9830):1984-96. DOI: 10.1016/S0140-6736(11)61346-9. View

Chen Y, Bao C, Zhang X, Lin X, Huang H, Wang Z . Long non-coding RNA HCG11 modulates glioma progression through cooperating with miR-496/CPEB3 axis. Cell Prolif. 2019; 52(5):e12615. PMC: 6797506. DOI: 10.1111/cpr.12615. View

Gutschner T, Hammerle M, Eissmann M, Hsu J, Kim Y, Hung G . The noncoding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells. Cancer Res. 2012; 73(3):1180-9. PMC: 3589741. DOI: 10.1158/0008-5472.CAN-12-2850. View

Jiang Q, Cheng L, Ma D, Zhao Y . FBXL19-AS1 exerts oncogenic function by sponging miR-431-5p to regulate RAF1 expression in lung cancer. Biosci Rep. 2019; 39(1). PMC: 6350044. DOI: 10.1042/BSR20181804. View

Lambert M, Jambon S, Depauw S, David-Cordonnier M . Targeting Transcription Factors for Cancer Treatment. Molecules. 2018; 23(6). PMC: 6100431. DOI: 10.3390/molecules23061479. View

Gao H, Liu R, Sun X . STAT3-induced upregulation of lncRNA SNHG17 predicts a poor prognosis of melanoma and promotes cell proliferation and metastasis through regulating PI3K-AKT pathway. Eur Rev Med Pharmacol Sci. 2019; 23(18):8000-8010. DOI: 10.26355/eurrev_201909_19016. View

10.

Lai N, Wu D, Fang X, Lin Y, Chen S, Li Z . Serum microRNA-210 as a potential noninvasive biomarker for the diagnosis and prognosis of glioma. Br J Cancer. 2015; 112(7):1241-6. PMC: 4385967. DOI: 10.1038/bjc.2015.91. View

11.

Niehrs C . The complex world of WNT receptor signalling. Nat Rev Mol Cell Biol. 2012; 13(12):767-79. DOI: 10.1038/nrm3470. View

12.

Tian J, Cao L, Dong G . Long noncoding RNA DDX11-AS1 induced by YY1 accelerates colorectal cancer progression through targeting miR-873/CLDN7 axis. Eur Rev Med Pharmacol Sci. 2019; 23(13):5714-5729. DOI: 10.26355/eurrev_201907_18309. View

13.

Louis D, Perry A, Reifenberger G, von Deimling A, Figarella-Branger D, Cavenee W . The 2016 World Health Organization Classification of Tumors of the Central Nervous System: a summary. Acta Neuropathol. 2016; 131(6):803-20. DOI: 10.1007/s00401-016-1545-1. View

14.

Lan T, Yuan K, Yan X, Xu L, Liao H, Hao X . LncRNA SNHG10 Facilitates Hepatocarcinogenesis and Metastasis by Modulating Its Homolog SCARNA13 via a Positive Feedback Loop. Cancer Res. 2019; 79(13):3220-3234. DOI: 10.1158/0008-5472.CAN-18-4044. View

15.

Fu C, Li D, Zhang X, Liu N, Chi G, Jin X . LncRNA PVT1 Facilitates Tumorigenesis and Progression of Glioma via Regulation of MiR-128-3p/GREM1 Axis and BMP Signaling Pathway. Neurotherapeutics. 2018; 15(4):1139-1157. PMC: 6277294. DOI: 10.1007/s13311-018-0649-9. View

16.

Hu Y, Kang C, Zhao J, Nie Y, Zheng L, Li H . LncRNA PLAC2 down-regulates RPL36 expression and blocks cell cycle progression in glioma through a mechanism involving STAT1. J Cell Mol Med. 2017; 22(1):497-510. PMC: 5742712. DOI: 10.1111/jcmm.13338. View

17.

Mansouri A, Mansouri S, Hachem L, Klironomos G, Vogelbaum M, Bernstein M . The role of 5-aminolevulinic acid in enhancing surgery for high-grade glioma, its current boundaries, and future perspectives: A systematic review. Cancer. 2016; 122(16):2469-78. DOI: 10.1002/cncr.30088. View

18.

Anastas J, Moon R . WNT signalling pathways as therapeutic targets in cancer. Nat Rev Cancer. 2012; 13(1):11-26. DOI: 10.1038/nrc3419. View

19.

Zhang L, Zhang Y . Tunneling nanotubes between rat primary astrocytes and C6 glioma cells alter proliferation potential of glioma cells. Neurosci Bull. 2015; 31(3):371-8. PMC: 5563692. DOI: 10.1007/s12264-014-1522-4. View

20.

Patel J, Hu M, Sinha G, Walker N, Sherman L, Gallagher A . Non-coding RNA as mediators in microenvironment-breast cancer cell communication. Cancer Lett. 2015; 380(1):289-95. DOI: 10.1016/j.canlet.2015.11.016. View