The 5.8S Pre-rRNA Maturation Factor, M-phase Phosphoprotein 6, is a Female Fertility Factor Required for Oocyte Quality and Meiosis

Overview

Journal Cell Prolif

Date 2020 Feb 1

PMID 32003502

Citations 1

Authors

Rui-Rui Peng

Li-Li Wang

Wen-Yi Gao

Feng-Yu Zhu

Fan Hu

Wen-Tao Zeng

Li-Ya Shi

Xi-Chen Chen

Jing-Yang Cai

Dong Zhang

Zheng-Rong Xia

Zhi-Xia Yang

Affiliations

Soon will be listed here.

Abstract

Objectives: M-phase phosphoprotein 6 (MPP6) is important for 5.8S pre-rRNA maturation in somatic cells and was screened as a female fertility factor. However, whether MPP6 functions in oocyte meiosis and fertility is not yet known. We aimed to address this.

Materials And Methods: Mouse oocytes with surrounded nucleus (SN) or non-surrounded nucleus (NSN) were used for all experiments. Peptide nanoparticle-mediated antibody transfection was used to deplete MPP6. Immunofluorescence staining, immunohistochemistry and live tracker staining were used to examine MPP6 localization and characterize phenotypes after control or MPP6 depletion. High-fidelity PCR and fluorescence in situ hybridization (FISH) were used to examine the localization and level of 5.8S rRNAs. Western blot was used to examine the protein level. MPP6-EGFP mRNA microinjection was used to do the rescue.

Results: MPP6 was enriched within ovaries and oocytes. MPP6 depletion significantly impeded oocyte meiosis. MPP6 depletion increased 5.8S pre-rRNA. The mRNA levels of MPP6 and 5.8S rRNA decreased within ageing oocytes, and MPP6 mRNA injection partially increased 5.8S rRNA maturation and improved oocyte quality.

Conclusions: MPP6 is required for 5.8S rRNA maturation, meiosis and quality control in mouse oocytes, and MPP6 level might be a marker for oocyte quality.

Citing Articles

The 5.8S pre-rRNA maturation factor, M-phase phosphoprotein 6, is a female fertility factor required for oocyte quality and meiosis.

Peng R, Wang L, Gao W, Zhu F, Hu F, Zeng W Cell Prolif. 2020; 53(3):e12769.

PMID: 32003502 PMC: 7106954. DOI: 10.1111/cpr.12769.

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