A Platform for Studying of the Three-Dimensional Migration of Hematopoietic Stem/Progenitor Cells

Overview

Journal Tissue Eng Regen Med

Date 2020 Feb 1

PMID 32002840

Citations 1

Authors

Eunjin Lee

Jieun Kim

Yungyeong Kang

Jung-Woog Shin

Affiliations

Soon will be listed here.

Abstract

Background: Hematopoietic stem/progenitor cells (HSPCs) have the property to return to the bone marrow, which is believed to be critical in situations such as HSPC transplantation. This property plays an important role in the stemness, viability, and proliferation of HSPCs, also. However, most in vitro models so far have not sufficiently simulated the complicate environment. Here, we proposed a three-dimensional experimental platform for the quantitative study of the migration of HSPCs.

Methods: After encapsulating osteoblasts (OBs) in alginate beads, we quantified the migration of HSPCs into the beads due to the physical environment using digital image processing. Intermittent hydrostatic pressure (IHP) was used to mimic the mechanical environment of human bone marrow without using any biochemical factors. The expression of stromal cell-derived factor 1 (SDF-1) under IHP was measured.

Results: The results showed that the presence of OBs in the hydrogel scaffold initiate the movement of HSPCs. Furthermore, the IHP promotes the migration of HSPCs, even without the addition of any biochemical factors, and the results were confirmed by measuring SDF-1 levels.

Conclusion: We believe this suggested three-dimensional experimental platform consisting of a simulated in vivo physical environment and encapsulated OBs should contribute to in vitro migration studies used to investigate the effects of other external factors.

Citing Articles

Biomechanical cues as master regulators of hematopoietic stem cell fate.

Li H, Luo Q, Shan W, Cai S, Tie R, Xu Y Cell Mol Life Sci. 2021; 78(16):5881-5902.

PMID: 34232331 PMC: 8316214. DOI: 10.1007/s00018-021-03882-y.

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