High-level Extracellular Production of an Alkaline Pectate Lyase in BL21 (DE3) and Its Application in Bioscouring of Cotton Fabric
Overview
Authors
Affiliations
A high heterologous expression of an alkaline pectate lyase (APL) pelNK93I in was obtained through optimizing the lactose feeding and fed-batch fermentation. The highest soluble APL activity produced by BL21 (pET22b-) was 10,181 U/mL which is the highest level so far. On this basis, to improve the extracellular yield of APL, optimized glycine feeding was used to achieve elevated extracellular production of pelNK93I. The highest extracellular APL activity produced by BL21 (pET22b-) was 6357 U/mL which was also relatively higher than that in previous reports. The final productivity of APL was 282.8 U/mL/h in the fermentation of BL21 (pET22b-3I) in a 10 L fermenter. Thus the current study has provided a cost-effective method for the over-expression and preparation of alkaline pectate lyase pelNK93I for its industrial applications. Moreover, pelNK93I (4 U/mL) used for bioscouring increased cottonseed husk removal and radial capillary effect of cotton fabric by 37.63% and 47.06%, respectively, making it a promising enzyme in green textile technology.
Zhang M, Zhen J, Teng J, Zhao X, Fu X, Song H Microorganisms. 2024; 12(7).
PMID: 39065044 PMC: 11278945. DOI: 10.3390/microorganisms12071275.
Zhang M, Fu X, Gu R, Zhao B, Zhao X, Song H BMC Biotechnol. 2024; 24(1):2.
PMID: 38200466 PMC: 10782670. DOI: 10.1186/s12896-023-00826-1.
Improvement of optimum pH and specific activity of pectate lyase from RN.1 using loop replacement.
Li P, Wei X, Wang Y, Liu H, Xu Y, Zhang Z Front Bioeng Biotechnol. 2023; 11:1242123.
PMID: 37469444 PMC: 10352616. DOI: 10.3389/fbioe.2023.1242123.
Liu J, Liu C, Qiao S, Dong Z, Sun D, Zhu J BMC Biotechnol. 2022; 22(1):6.
PMID: 35123477 PMC: 8817556. DOI: 10.1186/s12896-022-00736-8.
Satapathy S, Rout J, Kerry R, Thatoi H, Sahoo S Front Nutr. 2020; 7:117.
PMID: 32850938 PMC: 7424017. DOI: 10.3389/fnut.2020.00117.