Evaluation of Cytotoxicity, Cell Cycle Arrest and Apoptosis Induced by L Essential Oil in Human Hepatocellular Carcinoma Cell Line

Overview

Journal Saudi Pharm J

Specialty Pharmacy

Date 2020 Jan 31

PMID 31997913

Citations 11

Authors

Ebtesam S Al-Sheddi

Nouf A Al-Zaid

Mai M Al-Oqail

Shaza M Al-Massarani

Ali A El-Gamal

Nida N Farshori

Affiliations

Soon will be listed here.

Abstract

L. () commonly known as , is an essential oil bearing plant extensively being used in traditional system of medicine. However, the reports on the components and biological responses of essential oil (AG-EO) from Saudi Arabia are scarce. The present study was designed to explore the presence of basic constituents and apoptosis induced by AG-EO in HepG2 cells. The constituents in AG-EO was analyzed by Gas chromatography-Mass spectroscopy (GC-MS). Cytotoxicity of AG-EO was measured by MTT assay and cell cycle arrest and apoptosis assays were conducted by using flow cytometer. Based on GC-MS analysis, the main constituents present in AG-EO were carvone (53.130%), dillapole (25.420%), dihydrocarvone 2 (11.350%) and dihydrocarvone 1 (6.260%). A few other minor components were also identified -dihydrocarveol (0.690%), limonene (0.580%), isodihydrocarveol (0.370%), myristicin (0.210%) and -arsone (0.190%). The cytotoxicity results showed that AG-EO decrease the cell viability and inhibit the cell growth of HepG2 cells in a concentration-dependent manner. The inhibitory activity of AG-EO was found with IC = 59.6  5.64. The cell cycle arrest results showed that HepG2 cells exposed to AG-EO exhibited an increase in G2/M and pre-G1 cell population after 24 h exposure. Furthermore, the flow cytometry data revealed the primarily activation of cell death by apoptosis manners in HepG2 cells exposed to AG-EO. Overall, results from this study highlighted the anticancer potential of AG-EO, which could be considered as a new agent for the management of hepatocellular carcinoma.

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References

Lazutka J, Mierauskiene J, Slapsyte G, Dedonyte V . Genotoxicity of dill (Anethum graveolens L.), peppermint (Menthaxpiperita L.) and pine (Pinus sylvestris L.) essential oils in human lymphocytes and Drosophila melanogaster. Food Chem Toxicol. 2001; 39(5):485-92. DOI: 10.1016/s0278-6915(00)00157-5. View

Keyvani-Ghamsari S, Rabbani-Chadegani A, Sargolzaei J, Shahhoseini M . Effect of irinotecan on HMGB1, MMP9 expression, cell cycle, and cell growth in breast cancer (MCF-7) cells. Tumour Biol. 2017; 39(4):1010428317698354. DOI: 10.1177/1010428317698354. View

Palchaudhuri R, Hergenrother P . DNA as a target for anticancer compounds: methods to determine the mode of binding and the mechanism of action. Curr Opin Biotechnol. 2007; 18(6):497-503. DOI: 10.1016/j.copbio.2007.09.006. View

Joe A, Liu H, Suzui M, Vural M, Xiao D, Weinstein I . Resveratrol induces growth inhibition, S-phase arrest, apoptosis, and changes in biomarker expression in several human cancer cell lines. Clin Cancer Res. 2002; 8(3):893-903. View

Elmore S . Apoptosis: a review of programmed cell death. Toxicol Pathol. 2007; 35(4):495-516. PMC: 2117903. DOI: 10.1080/01926230701320337. View

Al-Sheddi E, Al-Oqail M, Saquib Q, Siddiqui M, Musarrat J, Ali Al-Khedhairy A . Novel all trans-retinoic Acid derivatives: cytotoxicity, inhibition of cell cycle progression and induction of apoptosis in human cancer cell lines. Molecules. 2015; 20(5):8181-97. PMC: 6272518. DOI: 10.3390/molecules20058181. View

Jamali T, Kavoosi G, Safavi M, Ardestani S . In-vitro evaluation of apoptotic effect of OEO and thymol in 2D and 3D cell cultures and the study of their interaction mode with DNA. Sci Rep. 2018; 8(1):15787. PMC: 6202332. DOI: 10.1038/s41598-018-34055-w. View

OPDYKE D, Letizia C . Monographs on fragrance raw materials. Food Chem Toxicol. 1982; 20 Suppl:633-852. DOI: 10.1016/0015-6264(77)90086-4. View

Chang W, Cheng F, Wang S, Chou S, Shih Y . Cinnamomum cassia essential oil and its major constituent cinnamaldehyde induced cell cycle arrest and apoptosis in human oral squamous cell carcinoma HSC-3 cells. Environ Toxicol. 2016; 32(2):456-468. DOI: 10.1002/tox.22250. View

10.

Zheng G, Kenney P, Lam L . Anethofuran, carvone, and limonene: potential cancer chemopreventive agents from dill weed oil and caraway oil. Planta Med. 1992; 58(4):338-41. DOI: 10.1055/s-2006-961480. View

11.

Al-Oqail M, Farshori N, Al-Sheddi E, Musarrat J, Ali Al-Khedhairy A, Siddiqui M . In vitro cytotoxic activity of seed oil of fenugreek against various cancer cell lines. Asian Pac J Cancer Prev. 2013; 14(3):1829-32. DOI: 10.7314/apjcp.2013.14.3.1829. View

12.

Elsayed E, Sharaf-Eldin M, Wadaan M . In vitro Evaluation of Cytotoxic Activities of Essential Oil from Moringa oleifera Seeds on HeLa, HepG2, MCF-7, CACO-2 and L929 Cell Lines. Asian Pac J Cancer Prev. 2015; 16(11):4671-5. DOI: 10.7314/apjcp.2015.16.11.4671. View

13.

Ishikawa T, Kudo M, Kitajima J . Water-soluble constituents of dill. Chem Pharm Bull (Tokyo). 2002; 50(4):501-7. DOI: 10.1248/cpb.50.501. View

14.

Maioli E, Torricelli C, Fortino V, Carlucci F, Tommassini V, Pacini A . Critical appraisal of the MTT assay in the presence of rottlerin and uncouplers. Biol Proced Online. 2009; 11:227-40. PMC: 3056051. DOI: 10.1007/s12575-009-9020-1. View

15.

Goun E, Petrichenko V, Solodnikov S, Suhinina T, Kline M, Cunningham G . Anticancer and antithrombin activity of Russian plants. J Ethnopharmacol. 2002; 81(3):337-42. DOI: 10.1016/s0378-8741(02)00116-2. View

16.

Goodarzi M, Khodadadi I, Tavilani H, Abbasi Oshaghi E . The Role of L. (Dill) in the Management of Diabetes. J Trop Med. 2016; 2016:1098916. PMC: 5088306. DOI: 10.1155/2016/1098916. View

17.

Srivastava S, Somasagara R, Hegde M, Nishana M, Tadi S, Srivastava M . Quercetin, a Natural Flavonoid Interacts with DNA, Arrests Cell Cycle and Causes Tumor Regression by Activating Mitochondrial Pathway of Apoptosis. Sci Rep. 2016; 6:24049. PMC: 4828642. DOI: 10.1038/srep24049. View

18.

Mosmann T . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65(1-2):55-63. DOI: 10.1016/0022-1759(83)90303-4. View

19.

Al-Asmari A, Al-Elaiwi A, Athar M, Tariq M, Al Eid A, Al-Asmary S . A review of hepatoprotective plants used in saudi traditional medicine. Evid Based Complement Alternat Med. 2015; 2014:890842. PMC: 4281445. DOI: 10.1155/2014/890842. View

20.

Naseri M, Mojab F, Khodadoost M, Kamalinejad M, Davati A, Choopani R . The Study of Anti-Inflammatory Activity of Oil-Based Dill (Anethum graveolens L.) Extract Used Topically in Formalin-Induced Inflammation Male Rat Paw. Iran J Pharm Res. 2013; 11(4):1169-74. PMC: 3813168. View