The Role of Regulatory T Cells in Allergic Rhinitis and Their Correlation with IL-10, IL-17 and Neopterin Levels in Serum and Nasal Lavage Fluid

Overview

Journal Eur Arch Otorhinolaryngol

Specialty Otorhinolaryngology

Date 2020 Jan 30

PMID 31993765

Citations 9

Authors

Kadriye Erkan

Mete K Bozkurt

Hasibe Artac

Hulya Ozdemir

Ali Unlu

Emine N Korucu

Cagdas Elsurer

Affiliations

Soon will be listed here.

Abstract

Purpose: Allergic rhinitis (AR), is an IgE-mediated inflammation of the nose. Regulatory T cells (Tregs) and inflammatory cytokines have been shown to play a critical role in allergic airway inflammation. The aim of the study was to compare the levels of blood T lymphocyte subsets and IL-10, IL-17 and neopterin concentrations in serum and nasal lavage of patients with AR compared to healthy subjects.

Methods: The study included 38 subjects with moderate-severe AR and 36 sex- and age-matched controls. Peripheral blood CD3+, CD3+CD4+ and CD4+CD25+Foxp3 percentages were evaluated using flow cytometry. Levels of IL-10, IL-17 and neopterin were measured both in serum and nasal lavage fluid with ELISA and HPLC, respectively.

Results: No difference was found in the percentages of T lymphocyte subsets between the two groups (p > 0.05). Serum IL-10 levels were similar (p > 0.05), whereas nasal IL-10 was lower in AR subjects compared to control group (2.22 ± 0.91 and 3.12 ± 1.45 pg/ml, respectively) (p < 0.05). Mean serum and nasal IL-17 were higher in AR (107.7 ± 79.61 and 527.36 ± 738.7 pg/ml) than the control group (76.29 ± 28.94 and 328.9 ± 430.8 pg/ml) (p < 0.05 and p > 0.05). There were no significant differences in serum and nasal neopterin levels (p > 0.05).

Conclusions: Although there were no differences in the distribution of lymphocyte subsets between the AR and control groups, the finding of higher levels of serum and nasal IL-17 and lower levels of nasal IL-10 support the cytokine imbalance in the pathogenesis of AR.

Citing Articles

Profile of immune response during nasal challenge with dermatophagoides pteronyssinus in subjects with allergic airway diseases.

Tamasauskiene L, Gradauskiene B J Inflamm (Lond). 2024; 21(1):41.

PMID: 39482718 PMC: 11526524. DOI: 10.1186/s12950-024-00415-9.

IL-10 inhibition during immunization improves vaccine-induced protection against Staphylococcus aureus infection.

Kelly A, McCarthy K, Claxton T, Carlile S, OBrien E, Vozza E JCI Insight. 2024; 9(13).

PMID: 38973612 PMC: 11383370. DOI: 10.1172/jci.insight.178216.

The Impact of Cytokines on Health-Related Quality of Life in Adolescents with Allergic Rhinitis.

Krsmanovic L, Arsovic N, Bokonjic D, Nesic V, Dudvarski Z, Pavlovic D Biomedicines. 2024; 12(2).

PMID: 38398030 PMC: 10886792. DOI: 10.3390/biomedicines12020428.

A Novel Strategy for the Treatment of Allergic Rhinitis: Regulating Treg/Th17 and Th1/Th2 Balance In Vivo by Vitamin D.

Li B, Zhang X, Sun Z, Xu B, Wu J, Liu H Comput Math Methods Med. 2022; 2022:9249627.

PMID: 35959353 PMC: 9357782. DOI: 10.1155/2022/9249627.

Therapeutic effect of Biyuan tongqiao granules combined with mometasone furoate nasal spray on clinical symptoms of children with allergic rhinitis.

Ding Z, Yang N, Cui X, Zhou D Am J Transl Res. 2022; 14(7):5116-5123.

PMID: 35958511 PMC: 9360867.

References

Li C, Lu H, Chen D, Lin Z, Wang D, Li T . In vivo and in vitro studies of Th17 response to specific immunotherapy in house dust mite-induced allergic rhinitis patients. PLoS One. 2014; 9(3):e91950. PMC: 3960160. DOI: 10.1371/journal.pone.0091950. View

Shi H, Li S, Xie Z, Qin X, Qin X, Zhong X . Regulatory CD4+CD25+ T lymphocytes in peripheral blood from patients with atopic asthma. Clin Immunol. 2004; 113(2):172-8. DOI: 10.1016/j.clim.2004.06.009. View

Jeannin P, Lecoanet S, Delneste Y, Gauchat J, Bonnefoy J . IgE versus IgG4 production can be differentially regulated by IL-10. J Immunol. 1998; 160(7):3555-61. View

Murr C, Widner B, Wirleitner B, Fuchs D . Neopterin as a marker for immune system activation. Curr Drug Metab. 2002; 3(2):175-87. DOI: 10.2174/1389200024605082. View

Palomares O, Martin-Fontecha M, Lauener R, Traidl-Hoffmann C, Cavkaytar O, Akdis M . Regulatory T cells and immune regulation of allergic diseases: roles of IL-10 and TGF-β. Genes Immun. 2014; 15(8):511-20. DOI: 10.1038/gene.2014.45. View

Nagalakshmi M, Murphy E, Mcclanahan T, De Waal Malefyt R . Expression patterns of IL-10 ligand and receptor gene families provide leads for biological characterization. Int Immunopharmacol. 2004; 4(5):577-92. DOI: 10.1016/j.intimp.2004.01.007. View

Bellinghausen I, Klostermann B, Knop J, Saloga J . Human CD4+CD25+ T cells derived from the majority of atopic donors are able to suppress TH1 and TH2 cytokine production. J Allergy Clin Immunol. 2003; 111(4):862-8. DOI: 10.1067/mai.2003.1412. View

Tsvetkova-Vicheva V, Gecheva S, Komsa-Penkova R, Velkova A, Lukanov T . IL-17 producing T cells correlate with polysensitization but not with bronchial hyperresponsiveness in patients with allergic rhinitis. Clin Transl Allergy. 2014; 4(1):3. PMC: 3896670. DOI: 10.1186/2045-7022-4-3. View

Huber C, Batchelor J, Fuchs D, Hausen A, Lang A, Niederwieser D . Immune response-associated production of neopterin. Release from macrophages primarily under control of interferon-gamma. J Exp Med. 1984; 160(1):310-6. PMC: 2187425. DOI: 10.1084/jem.160.1.310. View

10.

Hori S, Nomura T, Sakaguchi S . Control of regulatory T cell development by the transcription factor Foxp3. Science. 2003; 299(5609):1057-61. DOI: 10.1126/science.1079490. View

11.

Akdis M, Verhagen J, Taylor A, Karamloo F, Karagiannidis C, Crameri R . Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cells. J Exp Med. 2004; 199(11):1567-75. PMC: 2211782. DOI: 10.1084/jem.20032058. View

12.

Grunig G, Corry D, Leach M, Seymour B, Kurup V, Rennick D . Interleukin-10 is a natural suppressor of cytokine production and inflammation in a murine model of allergic bronchopulmonary aspergillosis. J Exp Med. 1997; 185(6):1089-99. PMC: 2196229. DOI: 10.1084/jem.185.6.1089. View

13.

Lee J, Yu H, Wang L, Yang Y, Lin Y, Chiang B . The levels of CD4+CD25+ regulatory T cells in paediatric patients with allergic rhinitis and bronchial asthma. Clin Exp Immunol. 2007; 148(1):53-63. PMC: 1868849. DOI: 10.1111/j.1365-2249.2007.03329.x. View

14.

Wong C, Ho C, Ko F, Chan C, Ho A, Hui D . Proinflammatory cytokines (IL-17, IL-6, IL-18 and IL-12) and Th cytokines (IFN-gamma, IL-4, IL-10 and IL-13) in patients with allergic asthma. Clin Exp Immunol. 2001; 125(2):177-83. PMC: 1906135. DOI: 10.1046/j.1365-2249.2001.01602.x. View

15.

Meiler F, Zumkehr J, Klunker S, Ruckert B, Akdis C, Akdis M . In vivo switch to IL-10-secreting T regulatory cells in high dose allergen exposure. J Exp Med. 2008; 205(12):2887-98. PMC: 2585856. DOI: 10.1084/jem.20080193. View

16.

Akdis C, Blesken T, Akdis M, Wuthrich B, Blaser K . Role of interleukin 10 in specific immunotherapy. J Clin Invest. 1998; 102(1):98-106. PMC: 509070. DOI: 10.1172/JCI2250. View

17.

Akdis M, Burgler S, Crameri R, Eiwegger T, Fujita H, Gomez E . Interleukins, from 1 to 37, and interferon-γ: receptors, functions, and roles in diseases. J Allergy Clin Immunol. 2011; 127(3):701-21.e1-70. DOI: 10.1016/j.jaci.2010.11.050. View

18.

Enk A, Angeloni V, Udey M, Katz S . Inhibition of Langerhans cell antigen-presenting function by IL-10. A role for IL-10 in induction of tolerance. J Immunol. 1993; 151(5):2390-8. View

19.

Stelmaszczyk-Emmel A, Zawadzka-Krajewska A, Szypowska A, Kulus M, Demkow U . Frequency and activation of CD4+CD25 FoxP3+ regulatory T cells in peripheral blood from children with atopic allergy. Int Arch Allergy Immunol. 2013; 162(1):16-24. DOI: 10.1159/000350769. View

20.

Genc S, Eroglu H, Kucuksezer U, Aktas-Cetin E, Gelincik A, Ustyol-Aycan E . The decreased CD4+CD25+ FoxP3+ T cells in nonstimulated allergic rhinitis patients sensitized to house dust mites. J Asthma. 2012; 49(6):569-74. DOI: 10.3109/02770903.2012.695418. View