Effect of Different Mycobionts on Symbiotic Germination and Seedling Growth of Dendrobium Officinale, an Important Medicinal Orchid

Overview

Journal Bot Stud

Specialty Biology

Date 2020 Jan 29

PMID 31989371

Citations 16

Authors

Ying Zhang

Yuan-Yuan Li

Xiao-Mei Chen

Shun-Xing Guo

Yung-I Lee

Affiliations

Soon will be listed here.

Abstract

Background: Orchids maintain a symbiotic relationship with mycorrhizal fungi in the lifecycle. Previous reports indicated that diverse mycobionts may have different roles during orchid growth and development. Although various mycorrhizal fungi have been isolated from Dendrobium roots and protocorms, little is known about their specific effects on seed germination and seedling growth. To understand the specific role of isolated fungal strains (i.e., Tulasnella and Sebacina), we used symbiotic culture to compare the effect of 6 fungal strains on seed germination and seedling growth of Dendrobium officinale, an important Chinese medicinal orchid.

Results: In symbiotic germination tests, 6 fungal strains (4 Tulasnella strains and 2 Sebacina strains) promoted seed germination with different efficiencies. Seeds inoculated with Tulasnella strains S6 and S7 conferred higher germination percentage and faster protocorm development than other fungal strains. In symbiotic cultures, seedlings inoculated with Sebacina strain S3 had optimal fresh and dry matter yield. Also, Tulasnella strains S6 and S7 promoted seedling growth with good fresh and dry matter yield. Sebacina strain S2 inoculation greatly enhanced root and tiller production and the content of total crude polysaccharides, although seedlings were smaller with less fresh and dry matter yield than other seedlings.

Conclusions: Tulasnella and Sebacina strains could promote seed germination and seedling growth of D. officinale with different efficiencies. Our results suggest a non-specific mycorrhizal association and development-dependent preference. Our data provide the basic knowledge for use of different fungal strains in conservation and/or production practices of D. officinale.

Citing Articles

Dynamic Changes in Functional Components of Dendrobium officinale and Their Applications in Food Science: A Review.

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Roles of mycorrhizal fungi on seed germination of two Chinese medicinal orchids: need or do not need a fungus?.

Yang J, Li N, Gao J Front Plant Sci. 2024; 15:1415401.

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Perspective and challenges of mycorrhizal symbiosis in orchid medicinal plants.

Leng C, Hou M, Xing Y, Chen J Chin Herb Med. 2024; 16(2):172-179.

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Optimization of the selection of suitable harvesting periods for medicinal plants: taking Dendrobium officinale as an example.

Li P, Shen T, Li L, Wang Y Plant Methods. 2024; 20(1):43.

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