Results of the 2018 Rapid DNA Maturity Assessment

Overview

Journal J Forensic Sci

Specialty Forensic Sciences

Date 2020 Jan 28

PMID 31985834

Citations 5

Authors

Erica L Romsos

Julie L French

Mark Smith

Vincent Figarelli

Frederick Harran

Glenn Vandegrift

Lilliana I Moreno

Thomas F Callaghan

Joanie Brocato

Janaki Vaidyanathan

Juan C Pedroso

Andrea Amy

Stephanie Stoiloff

Victor H Morillo

Karina Czetyrko

Elizabeth D Johnson

Jessica de Tagyos

Ashley Murray

Peter M Vallone

Affiliations

Soon will be listed here.

Abstract

Three commercially available integrated rapid DNA instruments were tested as a part of a rapid DNA maturity assessment in July of 2018. The assessment was conducted with sets of blinded single-source reference samples provided to participants for testing on the individual rapid platforms within their laboratories. The data were returned to the National Institute of Standards and Technology (NIST) for review and analysis. Both FBI-defined automated review (Rapid DNA Analysis) and manual review (Modified Rapid DNA Analysis) of the datasets were conducted to assess the success of genotyping the 20 Combined DNA Index System (CODIS) core STR loci and full profiles generated by the instruments. Genotype results from the multiple platforms, participating laboratories, and STR typing chemistries were combined into a single analysis. The Rapid DNA Analysis resulted in a success rate of 80% for full profiles (85% for the 20 CODIS core loci) with automated analysis. Modified Rapid DNA Analysis resulted in a success rate of 90% for both the CODIS 20 core loci and full profiles (all attempted loci per chemistry). An analysis of the peak height ratios demonstrated that 95% of all heterozygous alleles were above 59% heterozygote balance. For base-pair sizing precision, the precision was below the standard 0.5 bp deviation for both the ANDE 6C System and the RapidHIT 200.

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