Gga-miR-30d Regulates Infectious Bronchitis Virus Infection by Targeting USP47 in HD11 Cells

Overview

Journal Microb Pathog

Specialties Infectious Diseases
Microbiology

Date 2020 Jan 27

PMID 31982568

Citations 11

Authors

Hao Li

Jianan Li

Yaru Zhai

Lan Zhang

Pengfei Cui

Lan Feng

Wenjun Yan

Xue Fu

Yiming Tian

Hongning Wang

Xin Yang

Affiliations

Soon will be listed here.

Abstract

Avian infectious bronchitis virus (IBV) is a coronavirus which infects chickens and causes severe economic losses to the poultry industry worldwide. MicroRNAs (miRNAs) are important intracellular regulators and play a pivotal role in viral infections. In previous studies, we have revealed that IBV infection caused a significant down-regulation of gga-miR-30d expression in chicken kidneys. In present study, we investigated the role of gga-miR-30d in the process of IBV infection of HD11 cell line in vitro. By transfecting the mimics and inhibitor of gga-miR-30d, it was found that overexpressed gga-miR-30d inhibited IBV replication. Contrarily, low-expressed gga-miR-30d promoted IBV replication. In addition, dual-luciferase reporter assays revealed that ubiquitin-specific protease 47 (USP47), a deubiquitinase-encoding gene, was a target for gga-miR-30d. This is the first study demonstrating that miRNAs regulate IBV replication by regulating the deubiquitinating enzyme (DUBs).

Citing Articles

Characterization of microRNA candidates at the primary site of infectious bronchitis virus infection: A comparative study of in vitro and in vivo avian models.

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Viral deubiquitinating proteases and the promising strategies of their inhibition.

van Vliet V, De Silva A, Mark B, Kikkert M Virus Res. 2024; 344:199368.

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miR-30d Inhibition Protects IPEC-J2 Cells Against Beta2 Toxin-Induced Inflammatory Injury.

Xie K, Yang Q, Yan Z, Gao X, Huang X, Wang P Front Vet Sci. 2022; 9:909500.

PMID: 35799836 PMC: 9253665. DOI: 10.3389/fvets.2022.909500.

The Role of Deubiquitinases in Virus Replication and Host Innate Immune Response.

Zhang Q, Jia Q, Gao W, Zhang W Front Microbiol. 2022; 13:839624.

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