Nitrogen Preferences During Alcoholic Fermentation of Different Non- Yeasts of Oenological Interest

Non- yeasts have long been considered spoilage microorganisms. Currently, oenological interest in those species is increasing, mostly due to their positive contribution to wine quality. In this work, the fermentative capacity and nitrogen consumption of several non- wine yeast ( and ) were analyzed. For this purpose, synthetic must with three different nitrogen compositions was used: a mixture of amino acids and ammonium, only organic or inorganic nitrogen. The fermentation kinetics, nitrogen consumption, and yeast growth were measured over time. Our results showed that the good fermentative strains, and , had high similarities with in terms of growth, fermentation profile, and nitrogen assimilation preferences, although presented an impaired behavior when only amino acids or ammonia were used, being strain-specific. was the non- strain least affected by the nitrogen composition of the medium. The other two poor fermentative strains, and , behaved similarly regarding amino acid uptake, which occurred earlier than that of the good fermentative species in the absence of ammonia. The results obtained in single non- fermentations highlighted the importance of controlling nitrogen requirements of the wine yeasts, mainly in sequential fermentations, in order to manage a proper nitrogen supplementation, when needed.