Vitamins and Other Metabolites in Various Sera Commonly Used for Cell Culturing
Overview
Authors
Affiliations
Many cell culture media use different sera to enhance growth. We assayed vitamins and some related metabolites in different sera and identified the concentration of: thiamin, biotin, folates, riboflavin, pantothenates, nicotinates, vitamins B6, B12, A, E, C, and carotenes and some related metabolites: biopterins, free inositol, free and total choline, total carnitines in chicken, horse, rabbit, goat, pig, calf, newborn calf, fetal calf and human sera. Results indicate that vitamin and metabolite content of different sera vary. Such variations could produce fluctuant effects on cell culturings if the metabolite content of the serum is not documented.
Wang M, Zhao K, Liu M, Wang M, Qiao Z, Yi S Protein Cell. 2022; 13(8):580-601.
PMID: 35147915 PMC: 9232672. DOI: 10.1007/s13238-021-00896-x.
Punia S, Juran B, Ali A, Schlicht E, Moore R, Sun Z BMC Gastroenterol. 2021; 21(1):149.
PMID: 33794792 PMC: 8017778. DOI: 10.1186/s12876-021-01741-5.
Karadjian M, Senger A, Essers C, Wilkesmann S, Heller R, Fellenberg J Cells. 2020; 9(4).
PMID: 32283663 PMC: 7226817. DOI: 10.3390/cells9040918.
Jones D, Alphey M, Wyllie S, Fairlamb A Mol Microbiol. 2012; 86(1):51-64.
PMID: 22857512 PMC: 3470933. DOI: 10.1111/j.1365-2958.2012.08189.x.
Complete in vitro life cycle of Trypanosoma congolense: development of genetic tools.
Coustou V, Guegan F, Plazolles N, Baltz T PLoS Negl Trop Dis. 2010; 4(3):e618.
PMID: 20209144 PMC: 2830455. DOI: 10.1371/journal.pntd.0000618.