Scaffold Vascularization Method Using an Adipose-derived Stem Cell (ASC)-seeded Scaffold Prefabricated with a Flow-through Pedicle

Overview

Journal Stem Cell Res Ther

Publisher Biomed Central

Date 2020 Jan 25

PMID 31973733

Citations 5

Authors

Tomasz Debski

Agata Kurzyk

Barbara Ostrowska

Juliusz Wysocki

Jakub Jaroszewicz

Wojciech Swieszkowski

Zygmunt Pojda

Affiliations

Soon will be listed here.

Abstract

Background: Vascularization is important for the clinical application of tissue engineered products. Both adipose-derived stem cells (ASCs) and surgical prefabrication can be used to induce angiogenesis in scaffolds. Our aim was to compare the angiogenic potential of ASC-seeded scaffolds combined with scaffold prefabrication with that of non-seeded, non-prefabricated scaffolds.

Methods: For prefabrication, functional blood vessels were introduced into the scaffold using a flow-through pedicle system. ASCs were isolated from rat fat deposits. Three-dimensional-printed cylindrical poly-ε-caprolactone scaffolds were fabricated by fused deposition modelling. Three groups, each containing six rats, were investigated by using non-seeded, ASC-seeded, and osteogenic induced ASC-seeded scaffolds. In each group, one rat was implanted with two scaffolds in the inguinal region. On the right side, a scaffold was implanted subcutaneously around the inferior epigastric vessels (classic prefabrication group). On the left side, the inferior epigastric vessels were placed inside the prefabricated scaffold in the flow-through pedicle system (flow-through prefabrication group). The vessel density and vascular architecture were examined histopathologically and by μCT imaging, respectively, at 2 months after implantation.

Results: The mean vessel densities were 10- and 5-fold higher in the ASC-seeded and osteogenic induced ASC-seeded scaffolds with flow-through prefabrication, respectively, than in the non-seeded classic prefabricated group (p < 0.001). μCT imaging revealed functional vessels within the scaffold.

Conclusion: ASC-seeded scaffolds with prefabrication showed significantly improved scaffold vasculogenesis and could be useful for application to tissue engineering products in the clinical settings.

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References

Kurzyk A, Debski T, Swieszkowski W, Pojda Z . Comparison of adipose stem cells sources from various locations of rat body for their application for seeding on polymer scaffolds. J Biomater Sci Polym Ed. 2019; 30(5):376-397. DOI: 10.1080/09205063.2019.1570433. View

Cross M, Claesson-Welsh L . FGF and VEGF function in angiogenesis: signalling pathways, biological responses and therapeutic inhibition. Trends Pharmacol Sci. 2001; 22(4):201-7. DOI: 10.1016/s0165-6147(00)01676-x. View

Carmeliet P . Mechanisms of angiogenesis and arteriogenesis. Nat Med. 2000; 6(4):389-95. DOI: 10.1038/74651. View

Strem B, Hicok K, Zhu M, Wulur I, Alfonso Z, Schreiber R . Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med. 2005; 54(3):132-41. DOI: 10.2302/kjm.54.132. View

Miranville A, Heeschen C, Sengenes C, Curat C, Busse R, Bouloumie A . Improvement of postnatal neovascularization by human adipose tissue-derived stem cells. Circulation. 2004; 110(3):349-55. DOI: 10.1161/01.CIR.0000135466.16823.D0. View

Deng M, Gu Y, Liu Z, Qi Y, Ma G, Kang N . Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh. Stem Cells Int. 2015; 2015:350718. PMC: 4464689. DOI: 10.1155/2015/350718. View

Bolland B, Kanczler J, Dunlop D, Oreffo R . Development of in vivo muCT evaluation of neovascularisation in tissue engineered bone constructs. Bone. 2008; 43(1):195-202. DOI: 10.1016/j.bone.2008.02.013. View

Abramsson A, Lindblom P, Betsholtz C . Endothelial and nonendothelial sources of PDGF-B regulate pericyte recruitment and influence vascular pattern formation in tumors. J Clin Invest. 2003; 112(8):1142-51. PMC: 213487. DOI: 10.1172/JCI18549. View

Mizutani R, Suzuki Y . X-ray microtomography in biology. Micron. 2011; 43(2-3):104-15. DOI: 10.1016/j.micron.2011.10.002. View

10.

Ferrara N . Role of vascular endothelial growth factor in regulation of physiological angiogenesis. Am J Physiol Cell Physiol. 2001; 280(6):C1358-66. DOI: 10.1152/ajpcell.2001.280.6.C1358. View

11.

Can Z, Apaydin I, Ercocen A, Demirseren M, Sabuncuoglu B . Prefabrication of a high-density porous polyethylene implant using a vascular induction technique. Ann Plast Surg. 1998; 41(3):264-9. DOI: 10.1097/00000637-199809000-00007. View

12.

Zhu M, Heydarkhan-Hagvall S, Hedrick M, Benhaim P, Zuk P . Manual isolation of adipose-derived stem cells from human lipoaspirates. J Vis Exp. 2013; (79):e50585. PMC: 3935774. DOI: 10.3791/50585. View

13.

Cai L, Johnstone B, Cook T, Liang Z, Traktuev D, Cornetta K . Suppression of hepatocyte growth factor production impairs the ability of adipose-derived stem cells to promote ischemic tissue revascularization. Stem Cells. 2007; 25(12):3234-43. DOI: 10.1634/stemcells.2007-0388. View

14.

Ren L, Ma D, Feng X, Mao T, Liu Y, Ding Y . A novel strategy for prefabrication of large and axially vascularized tissue engineered bone by using an arteriovenous loop. Med Hypotheses. 2008; 71(5):737-40. DOI: 10.1016/j.mehy.2008.06.032. View

15.

Bai F, Wang Z, Lu J, Liu J, Chen G, Lv R . The correlation between the internal structure and vascularization of controllable porous bioceramic materials in vivo: a quantitative study. Tissue Eng Part A. 2010; 16(12):3791-803. DOI: 10.1089/ten.TEA.2010.0148. View

16.

Niemeyer P, Fechner K, Milz S, Richter W, Suedkamp N, Mehlhorn A . Comparison of mesenchymal stem cells from bone marrow and adipose tissue for bone regeneration in a critical size defect of the sheep tibia and the influence of platelet-rich plasma. Biomaterials. 2010; 31(13):3572-9. DOI: 10.1016/j.biomaterials.2010.01.085. View

17.

Koike N, Fukumura D, Gralla O, Au P, Schechner J, Jain R . Tissue engineering: creation of long-lasting blood vessels. Nature. 2004; 428(6979):138-9. DOI: 10.1038/428138a. View

18.

Patel-Hett S, DAmore P . Signal transduction in vasculogenesis and developmental angiogenesis. Int J Dev Biol. 2011; 55(4-5):353-63. PMC: 4075038. DOI: 10.1387/ijdb.103213sp. View

19.

Jain R, Au P, Tam J, Duda D, Fukumura D . Engineering vascularized tissue. Nat Biotechnol. 2005; 23(7):821-3. DOI: 10.1038/nbt0705-821. View

20.

Maredziak M, Marycz K, Lewandowski D, Siudzinska A, Smieszek A . Static magnetic field enhances synthesis and secretion of membrane-derived microvesicles (MVs) rich in VEGF and BMP-2 in equine adipose-derived stromal cells (EqASCs)-a new approach in veterinary regenerative medicine. In Vitro Cell Dev Biol Anim. 2014; 51(3):230-40. PMC: 4368852. DOI: 10.1007/s11626-014-9828-0. View