Reduced CRHR-2 in Placental Tissues is Associated with the High DNA Methylation of CRHR-2 Promoter in Idiopathic Preterm Birth

Overview

Journal Int J Clin Exp Pathol

Specialty Pathology

Date 2020 Jan 23

PMID 31966828

Authors

Lei Li

Wei Cui

Yan Zhang

Yuanli Wang

Affiliations

Soon will be listed here.

Abstract

The present study was to explore the expression of CRHR-1 and CRHR-2 in the placentas of idiopathic preterm birth (PTB-I), and to identify the and methylation with the deregulated CRHR-1 and CRHR-2. Immunohistochemical staining, real-time quantitative PCR and western blot analysis were performed to examine the expression of CRHR-1 and CRHR-2. The methylation in promoter regions of the and genes were specifically was analyzed using MethyLight method. Results demonstrated that the CRHR-2 expression was reduced in both mRNA and protein levels in PBT-I placental tissues, associating with a high level methylation of gene in PTB-I placental specimens. experiments demonstrated that both CRHR-1 and CRHR-2 were downregulated, whereas the methylation of both and was upregulated in the hypoxia-treated human trophoblast-like JEG3 cells. In conclusion, CRHR-2 was downregulated in PTB-I in placenta tissues, in association with a high DNA methylation of promoter. Methylation inhibitor might be a promising agent for PTB-I treatment.

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