Mechanisms Governing the Pioneering and Redistribution Capabilities of the Non-classical Pioneer PU.1

Overview

Journal Nat Commun

Specialty Biology

Date 2020 Jan 23

PMID 31964861

Citations 67

Authors

Julia Minderjahn

Andreas Schmidt

Andreas Fuchs

Rudolf Schill

Johanna Raithel

Magda Babina

Christian Schmidl

Claudia Gebhard

Sandra Schmidhofer

Karina Mendes

Anna Ratermann

Dagmar Glatz

Margit Nutzel

Matthias Edinger

Petra Hoffmann

Rainer Spang

Gernot Langst

Axel Imhof

Michael Rehli

Affiliations

Soon will be listed here.

Abstract

Establishing gene regulatory networks during differentiation or reprogramming requires master or pioneer transcription factors (TFs) such as PU.1, a prototype master TF of hematopoietic lineage differentiation. To systematically determine molecular features that control its activity, here we analyze DNA-binding in vitro and genome-wide in vivo across different cell types with native or ectopic PU.1 expression. Although PU.1, in contrast to classical pioneer factors, is unable to access nucleosomal target sites in vitro, ectopic induction of PU.1 leads to the extensive remodeling of chromatin and redistribution of partner TFs. De novo chromatin access, stable binding, and redistribution of partner TFs both require PU.1's N-terminal acidic activation domain and its ability to recruit SWI/SNF remodeling complexes, suggesting that the latter may collect and distribute co-associated TFs in conjunction with the non-classical pioneer TF PU.1.

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