Metabolic Rewiring of Synthetic Pyruvate Dehydrogenase Bypasses for Acetone Production in Cyanobacteria

Overview

Journal Plant Biotechnol J

Specialties Biology
Biotechnology

Date 2020 Jan 22

PMID 31960579

Citations 8

Authors

Hyun Jeong Lee

Jigyeong Son

Sang Jun Sim

Han Min Woo

Affiliations

Soon will be listed here.

Abstract

Designing synthetic pathways for efficient CO fixation and conversion is essential for sustainable chemical production. Here we have designed a synthetic acetate-acetyl-CoA/malonyl-CoA (AAM) bypass to overcome an enzymatic activity of pyruvate dehydrogenase complex. This synthetic pathway utilizes acetate assimilation and carbon rearrangements using a methyl malonyl-CoA carboxyltransferase. We demonstrated direct conversion of CO into acetyl-CoA-derived acetone as an example in photosynthetic Synechococcus elongatus PCC 7942 by increasing the acetyl-CoA pools. The engineered cyanobacterial strain with the AAM-bypass produced 0.41 g/L of acetone at 0.71 m/day of molar productivity. This work clearly shows that the synthetic pyruvate dehydrogenase bypass (AAM-bypass) is a key factor for the high-level production of an acetyl-CoA-derived chemical in photosynthetic organisms.

Citing Articles

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Chen A, Ku J, Tsai T, Hung J, Hung B, Lan E Adv Biochem Eng Biotechnol. 2023; 183:105-144.

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