An Eighth Complementation Group of Rodent Cells Hypersensitive to Ultraviolet Radiation

Overview

Journal Somat Cell Mol Genet

Specialties Cell Biology
Molecular Biology

Date 1988 Nov 1

PMID 3194799

Citations 17

Authors

L H Thompson

T Shiomi

E P Salazar

S A Stewart

Affiliations

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Abstract

Two mutant lines (US31, US46) of mouse lymphoma cells that are hypersensitive to ultraviolet (UV) radiation were previously found to belong to different complementation groups. The mutants were tested for their ability to complement the six known complementation groups of UV-sensitive Chinese hamster ovary (CHO) cells, which are defective in nucleotide excision repair, as well as a seventh group represented by a V79 mutant. Hybrid cells were produced by fusion with polyethylene glycol and tested in situ for UV resistance. The mouse mutant US46 complemented all CHO mutants except UV61. Therefore, US46 is assigned to the same complementation group as UV61, and it is probably defective in the same locus. The mouse mutant US31 produced UV-resistant hybrid cells in each of the seven crosses, indicating that it forms an eighth complementation group among the rodent mutants. Thus, at least eight genes are likely required to repair UV damage in rodent cells.

Citing Articles

Rodent UV-sensitive mutant cell lines in complementation groups 6-10 have normal general excision repair activity.

Reardon J, Thompson L, Sancar A Nucleic Acids Res. 1997; 25(5):1015-21.

PMID: 9023113 PMC: 146541. DOI: 10.1093/nar/25.5.1015.

Structure and expression of the excision repair gene ERCC6, involved in the human disorder Cockayne's syndrome group B.

Troelstra C, Hesen W, BOOTSMA D, Hoeijmakers J Nucleic Acids Res. 1993; 21(3):419-26.

PMID: 8382798 PMC: 309134. DOI: 10.1093/nar/21.3.419.

Evidence for a repair enzyme complex involving ERCC1 and complementing activities of ERCC4, ERCC11 and xeroderma pigmentosum group F.

van Vuuren A, Appeldoorn E, Odijk H, Yasui A, Jaspers N, BOOTSMA D EMBO J. 1993; 12(9):3693-701.

PMID: 8253091 PMC: 413646. DOI: 10.1002/j.1460-2075.1993.tb06044.x.

UVs syndrome, a new general category of photosensitive disorder with defective DNA repair, is distinct from xeroderma pigmentosum variant and rodent complementation group I.

Itoh T, Fujiwara Y, Ono T, Yamaizumi M Am J Hum Genet. 1995; 56(6):1267-76.

PMID: 7539208 PMC: 1801097.

Human chromosome 15 confers partial complementation of phenotypes to xeroderma pigmentosum group F cells.

Saxon P, Schultz R, Stanbridge E, Friedberg E Am J Hum Genet. 1989; 44(4):474-85.

PMID: 2929593 PMC: 1715561.