Micronucleus and Its Significance in Effusion Fluids

Overview

Journal J Cytol

Specialty Cell Biology

Date 2020 Jan 17

PMID 31942100

Citations 2

Authors

Dravya Jayakumar

Kalpana Kumari Kasturi

Affiliations

Soon will be listed here.

Abstract

Background: Micronucleus (MN) is an extranuclear body within the cell formed due to failure of incorporation of whole chromosomes or their fragments during cell division. MN scoring can be done to identify malignant effusions.

Aims: This study aimed to score micronuclei to distinguish malignant effusion from benign effusions and to correlate MN score with type of malignant effusion.

Methods And Materials: A retrospective study was conducted on 30 malignant and 30 benign effusions. The number of micronucleated cells per 1,000 cells was counted in effusion smears stained with Papanicolaou stain under oil immersion (1,000×).

Results: The mean MN score in malignant effusions was 3.77 with standard deviation (SD) of 2.13. The mean MN score in benign effusions was 0.50 with SD of 0.57. The difference in MN score between malignant and benign effusions is statistically significant ( < 0.001). A cut-off MN score of 6.5 was seen to distinguish malignant and benign effusions with 100% specificity and 100% sensitivity in this study.

Conclusions: MN score is higher in malignant effusions when compared with benign effusions. This can be used to differentiate malignant effusions from benign effusions in low resource setting.

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References

Durante M, Formenti S . Radiation-Induced Chromosomal Aberrations and Immunotherapy: Micronuclei, Cytosolic DNA, and Interferon-Production Pathway. Front Oncol. 2018; 8:192. PMC: 5992419. DOI: 10.3389/fonc.2018.00192. View

Hogstedt B, Mitelman F . The interrelations of micronuclei, chromosomal instability, and mutational activity in acute non-lymphocytic leukemia--a hypothesis. Hereditas. 1981; 95(1):165-7. DOI: 10.1111/j.1601-5223.1981.tb01336.x. View

Mohanty S, Dey P . Serous effusions: diagnosis of malignancy beyond cytomorphology. An analytic review. Postgrad Med J. 2003; 79(936):569-74. PMC: 1742845. DOI: 10.1136/pmj.79.936.569. View

Light R, MacGregor M, LUCHSINGER P, BALL Jr W . Pleural effusions: the diagnostic separation of transudates and exudates. Ann Intern Med. 1972; 77(4):507-13. DOI: 10.7326/0003-4819-77-4-507. View

Sangisetty S, Miner T . Malignant ascites: A review of prognostic factors, pathophysiology and therapeutic measures. World J Gastrointest Surg. 2012; 4(4):87-95. PMC: 3351493. DOI: 10.4240/wjgs.v4.i4.87. View

Metgud R, Neelesh B . Effect of staining procedures on the results of micronucleus assay in the exfoliated buccal mucosal cells of smokers and nonsmokers: A pilot study. J Cancer Res Ther. 2018; 14(2):372-376. DOI: 10.4103/0973-1482.157351. View

Kaur J, Dey P . Micronucleus to distinguish adenocarcinoma from reactive mesothelial cell in effusion fluid. Diagn Cytopathol. 2009; 38(3):177-9. DOI: 10.1002/dc.21168. View

Mason M, Bedrossian C, Fahey C . Value of immunocytochemistry in the study of malignant effusions. Diagn Cytopathol. 1987; 3(3):215-21. DOI: 10.1002/dc.2840030308. View

Sears D, HAJDU S . The cytologic diagnosis of malignant neoplasms in pleural and peritoneal effusions. Acta Cytol. 1987; 31(2):85-97. View

10.

Ong K, Indumathi V, Poh W, Ong Y . The diagnostic yield of pleural fluid cytology in malignant pleural effusions. Singapore Med J. 2000; 41(1):19-23. View

11.

Dixit R, Agarwal K, Gokhroo A, Patil C, Meena M, Shah N . Diagnosis and management options in malignant pleural effusions. Lung India. 2017; 34(2):160-166. PMC: 5351359. DOI: 10.4103/0970-2113.201305. View

12.

Tyagi R, Dey P, Uppal R, Rajwanshi A . Analysis of morphological markers of chromosomal instability in ascitic fluid. Diagn Cytopathol. 2015; 43(10):855-8. DOI: 10.1002/dc.23249. View

13.

Ganesan N, Phansalkar M, Ambroise M, Varghese R . Validating Micronucleus Score in Effusion Fluids. J Cytol. 2017; 34(4):193-196. PMC: 5655655. DOI: 10.4103/JOC.JOC_178_16. View

14.

Assawasaksakul T, Boonsarngsuk V, Incharoen P . A comparative study of conventional cytology and cell block method in the diagnosis of pleural effusion. J Thorac Dis. 2017; 9(9):3161-3167. PMC: 5708377. DOI: 10.21037/jtd.2017.08.52. View

15.

Unal T, Coban I . Increased micronucleus count predicts malignant behavior in pleural effusion fluid. Turk J Med Sci. 2018; 48(2):354-360. DOI: 10.3906/sag-1711-63. View

16.

Karoo R, Lloyd T, Garcea G, Redway H, Robertson G . How valuable is ascitic cytology in the detection and management of malignancy?. Postgrad Med J. 2003; 79(931):292-4. PMC: 1742707. DOI: 10.1136/pmj.79.931.292. View

17.

Arul P, Shetty S, Masilamani S, Akshatha C, Naveen Kumar B . Evaluation of Micronucleus in Exfoliated Buccal Epithelial Cells Using Liquid-based Cytology Preparation in Petrol Station Workers. Indian J Med Paediatr Oncol. 2017; 38(3):273-276. PMC: 5686965. DOI: 10.4103/ijmpo.ijmpo_26_16. View

18.

Avlasevich S, Khanal S, Singh P, Torous D, Bemis J, Dertinger S . Flow cytometric method for scoring rat liver micronuclei with simultaneous assessments of hepatocyte proliferation. Environ Mol Mutagen. 2018; 59(3):176-187. PMC: 5854533. DOI: 10.1002/em.22168. View

19.

Bonassi S, Znaor A, Ceppi M, Lando C, Chang W, Holland N . An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans. Carcinogenesis. 2006; 28(3):625-31. DOI: 10.1093/carcin/bgl177. View

20.

Valdes L, Alvarez D, Valle J, Pose A, San Jose E . The etiology of pleural effusions in an area with high incidence of tuberculosis. Chest. 1996; 109(1):158-62. DOI: 10.1378/chest.109.1.158. View