Modulation of Motility, Adhesion to Polystyrene Surfaces, and Invasion of INT407 Cells by Quorum-Sensing Inhibition

Overview

Journal Microorganisms

Specialty Microbiology

Date 2020 Jan 17

PMID 31940805

Citations 22

Authors

Katarina Simunovic

Dina Ramic

Changyun Xu

Sonja Smole Mozina

Affiliations

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Abstract

is a major foodborne pathogen, and the LuxS-mediated quorum-sensing (QS) system influences its motility, biofilm formation, invasion, host colonization, and virulence. QS therefore represents a target for the control of . The aim of this study was to investigate the correlation of QS inhibition with changes in motility, adhesion to polystyrene surfaces, and adhesion to and invasion of INT407 cells. This was achieved by studying (i) the -deficient mutant and (ii) treatment of with 20 natural extracts as six essential oils, 11 ethanolic extracts, and three pure compounds. Compared to the wild-type, the Δ mutant showed decreased motility, adhesion to polystyrene surfaces, and invasion of INT407 cells. The anti-QS effects of the treatments ( = 15/20) were assayed using BB170 bioluminescence. Moderate positive correlation was shown between QS reduction and reduced motility (τ = 0.492, = 0.024), adhesion to polystyrene surfaces (τ = 0.419, = 0.008), and invasion ( = 0.394, = 0.068). The best overall effect was achieved with a (roseroot) extract, with 96% QS reduction, a 1.41 log (96%) decrease in adhesion to polystyrene surfaces, and an 82% decrease in invasion. We show that natural extracts can reduce motility, adhesion to polystyrene surfaces, and invasion of INT407 cells by through modulation of the LuxS (QS) system.

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