Paris Saponin H Suppresses Human Hepatocellular Carcinoma (HCC) by Inactivation of Wnt/β-catenin Pathway and

Overview

Journal Int J Clin Exp Pathol

Specialty Pathology

Date 2020 Jan 15

PMID 31934124

Citations 11

Authors

Tiezhu Chen

Juan Lin

Daxuan Tang

Mei Zhang

Feiyan Wen

Dan Xue

Hao Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death in the world. , also known as Chong-lou in China, is traditionally used as an anticancer medicine. Paris saponin H (Ps H) has been reported to be one potential antitumor active component from and shows cytotoxicity on tumor cells. However, the role of Ps H in HCC is not clear.

Methods: PLC/PRF/5 and Huh7 cells were exposed to Ps H. Cell viability, migration, and invasion were measured with CCK-8 assay, EMT and Transwell assay, respectively. Western blot was employed to detect the expression of cleaved caspase 3, E-cadherin, vimentin, β-catenin, p-GSK-3β and GSK-3β. Apoptosis was assessed by flow cytometry, and caspase 3 activity assay. For in vivo experiments, xenograft tumors were induced with PLC/PRF/5 cells.

Results: Ps H reduced cell viability and induced apoptosis in HCC cells in the dose-dependent manner; EMT and invasion were inhibited by Ps H. Ps H downregulated expression of β-catenin and p-GSK-3β; in addition, β-catenin silencing mediated Ps H-induced suppression of cell progression in PLC/PRF/5 cells. An administration of Ps H effectively suppressed the tumor growth in the HCC xenograft model .

Conclusion: Ps H suppresses HCC cell progression through downregulation of β-catenin , and inhibits xenograft tumor growth, suggesting Ps H is an attractive candidate for clinical therapy for HCC.

Citing Articles

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