Variant Sublineages of Human Papillomavirus Type 16 Predispose Women to Persistent Infection Characterized by a Sequence Analysis of the E6, L1, and LCR Regions

Overview

Journal Int J Clin Exp Pathol

Specialty Pathology

Date 2020 Jan 15

PMID 31933750

Citations 3

Authors

Binlie Yang

Lei Zhang

Ai Zhang

Aizhi Zhou

Jieyan Yuan

Yuhua Wang

Liyan Sun

Huimin Cao

Wenxin Zheng

Affiliations

Soon will be listed here.

Abstract

Background: One of the precursors of cervical cancer is persistent infection with human papillomavirus (HPV), especially high-risk HPV. The aim of this study was to verify the relationship between HPV16 variants and persistent viral infection.

Methods: Three-hundred and eighty-six Chinese women who had a low-grade squamous intraepithelial lesion (LSIL) or a lesion below LSIL with normal cellular morphology were selected and enrolled in this study. Flow-through hybridization and gene chip technology were applied to identify the HPV type, and a PCR-sequencing assay was performed to detect HPV16 , and long control region () gene variants. The relationship between HPV16 variants and persistent infection was analyzed using Fisher's exact test.

Results: In this population, 74.09% of HPV16 isolates belonged to the A4 sublineage, 24.87% to the A1/A2 sublineages, and 3.13% to B1/B2 sublineages. In addition, the A4 sublineage T178G (P<0.001) and the A1/A2 sublineages T350G and A442C (P<0.001) were associated with persistent HPV16 infection. and variants were found to be common in this population. Nonetheless, no significant relation was identified between the or variants and the persistence of infection (P>0.05).

Conclusion: HPV16 E6 variants in the Shanghai Pudong District mainly belong to the A4 sublineage, and detection of the specific HPV T178G genotype may be considered a risk factor for viral persistence and progression to other cervical diseases.

Citing Articles

Comparative Analysis of HPV16 Variants in the Untranslated Regulatory Region, L1, and E6 Genes among Vaccinated and Unvaccinated Young Women: Assessing Vaccine Efficacy and Viral Diversity.

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Wang X, Han S, Li X, Wang X, Wang S, Ma L Virol J. 2022; 19(1):37.

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