First Identification and Genotyping of Enterocytozoon Bieneusi in Humans in Myanmar

Overview

Journal BMC Microbiol

Publisher Biomed Central

Specialty Microbiology

Date 2020 Jan 15

PMID 31931704

Citations 11

Authors

Yujuan Shen

Baiyan Gong

Xiaohua Liu

Yanchen Wu

Fengkun Yang

Jie Xu

Xiaofan Zhang

Jianping Cao

Aiqin Liu

Affiliations

Soon will be listed here.

Abstract

Background: Intestinal pathogen infections are widespread among impoverished populations. Enterocytozoon bieneusi is the most common pathogen of intestinal microsporidian species in humans worldwide. However, no epidemiological information is available on E. bieneusi infection in humans in Myanmar. The present study comprised the first identification and genotyping of E. bieneusi in humans conducted in Myanmar.

Results: A total of 172 fecal specimens were collected from the Wa people (one each) in four villages of Pangsang Township of the Matman District of Shan State, Myanmar, and each participant completed a questionnaire. E. bieneusi was identified and genotyped using polymerase chain reaction (PCR) and sequence analysis of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. The average prevalence of E. bieneusi was 8.72% (15/172), ranging from 3.85 to 13.89% by village. E. bieneusi infection was not related to any of the risk factors studied. Six genotypes were identified, comprising two known genotypes Peru6 (n = 10) and D (n = 1) and four novel genotypes (MMR23, MMR25, MMR86, and MMR87) (one each), and two people infected with genotype Peru6 were from the same family. A phylogenetic analysis based on a neighbor-joining tree of the ITS sequences of E. bieneusi indicated that all the six genotypes were clustered into group 1.

Conclusions: This is the first identification and genotyping of E. bieneusi in humans in Myanmar. The observations that the two people infected with genotype Peru6 were from the same family, and that all six genotypes obtained in the present study fell into zoonotic group 1, showed the potential for anthropogenic and zoonotic transmissions. The present data argue for the importance of epidemiological control and prevention from medical sectors.

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References

Leelayoova S, Subrungruang I, Rangsin R, Chavalitshewinkoon-Petmitr P, Worapong J, Naaglor T . Transmission of Enterocytozoon bieneusi genotype a in a Thai orphanage. Am J Trop Med Hyg. 2005; 73(1):104-7. View

Aye T, Moe K, Nyein M, Swe T . Cryptosporidiosis in Myanmar infants with acute diarrhea. Southeast Asian J Trop Med Public Health. 1994; 25(4):654-6. View

Kim M, Jung B, Cho J, Kim D, Song H, Lee K . Prevalence of Intestinal Protozoans among Schoolchildren in Suburban Areas near Yangon, Myanmar. Korean J Parasitol. 2016; 54(3):345-8. PMC: 4977783. DOI: 10.3347/kjp.2016.54.3.345. View

Gong B, Yang Y, Liu X, Cao J, Xu M, Xu N . First survey of Enterocytozoon bieneusi and dominant genotype Peru6 among ethnic minority groups in southwestern China's Yunnan Province and assessment of risk factors. PLoS Negl Trop Dis. 2019; 13(5):e0007356. PMC: 6550416. DOI: 10.1371/journal.pntd.0007356. View

Al-Harazi T, Ghani M, Othman H . Prevalence of intestinal protozoan infections among Orang Asli schoolchildren in Pos Senderut, Pahang, Malaysia. J Egypt Soc Parasitol. 2014; 43(3):561-8. DOI: 10.12816/0006413. View

Lobo M, Xiao L, Antunes F, Matos O . Microsporidia as emerging pathogens and the implication for public health: a 10-year study on HIV-positive and -negative patients. Int J Parasitol. 2012; 42(2):197-205. DOI: 10.1016/j.ijpara.2011.12.002. View

Lee J . Molecular detection of Enterocytozoon bieneusi and identification of a potentially human-pathogenic genotype in milk. Appl Environ Microbiol. 2008; 74(5):1664-6. PMC: 2258614. DOI: 10.1128/AEM.02110-07. View

Liu H, Jiang Z, Yuan Z, Yin J, Wang Z, Yu B . Infection by and genotype characteristics of Enterocytozoon bieneusi in HIV/AIDS patients from Guangxi Zhuang autonomous region, China. BMC Infect Dis. 2017; 17(1):684. PMC: 5640944. DOI: 10.1186/s12879-017-2787-9. View

Wang L, Zhang H, Zhao X, Zhang L, Zhang G, Guo M . Zoonotic Cryptosporidium species and Enterocytozoon bieneusi genotypes in HIV-positive patients on antiretroviral therapy. J Clin Microbiol. 2012; 51(2):557-63. PMC: 3553929. DOI: 10.1128/JCM.02758-12. View

10.

Feng Y, Li N, Dearen T, Lobo M, Matos O, Cama V . Development of a multilocus sequence typing tool for high-resolution genotyping of Enterocytozoon bieneusi. Appl Environ Microbiol. 2011; 77(14):4822-8. PMC: 3147401. DOI: 10.1128/AEM.02803-10. View

11.

Satheesh Kumar S, Ananthan S, Joyee A . Detection of Enterocytozoon bieneusi (Microsporidia) by polymerase chain reaction (PCR) using species-specific primer in stool samples of HIV patients. Indian J Med Res. 2005; 121(4):215-9. View

12.

Decraene V, Lebbad M, Botero-Kleiven S, Gustavsson A, Lofdahl M . First reported foodborne outbreak associated with microsporidia, Sweden, October 2009. Epidemiol Infect. 2011; 140(3):519-27. PMC: 3267097. DOI: 10.1017/S095026881100077X. View

13.

Cama V, Pearson J, Cabrera L, Pacheco L, Gilman R, Meyer S . Transmission of Enterocytozoon bieneusi between a child and guinea pigs. J Clin Microbiol. 2007; 45(8):2708-10. PMC: 1951230. DOI: 10.1128/JCM.00725-07. View

14.

Stentiford G, Becnel -, Weiss L, Keeling P, Didier E, Williams B . Microsporidia - Emergent Pathogens in the Global Food Chain. Trends Parasitol. 2016; 32(4):336-348. PMC: 4818719. DOI: 10.1016/j.pt.2015.12.004. View

15.

Wanachiwanawin D, Manatsathit S, Lertlaituan P, Thakerngpol K, Suwanagool P . Intestinal microsporidiosis in HIV infected patients with chronic diarrhea in Thailand. Southeast Asian J Trop Med Public Health. 2000; 29(4):767-71. View

16.

Santin M, Fayer R . Enterocytozoon bieneusi genotype nomenclature based on the internal transcribed spacer sequence: a consensus. J Eukaryot Microbiol. 2009; 56(1):34-8. DOI: 10.1111/j.1550-7408.2008.00380.x. View

17.

Bern C, Kawai V, Vargas D, Rabke-Verani J, Williamson J, Chavez-Valdez R . The epidemiology of intestinal microsporidiosis in patients with HIV/AIDS in Lima, Peru. J Infect Dis. 2005; 191(10):1658-64. DOI: 10.1086/429674. View

18.

Ayinmode A, Ojuromi O, Xiao L . Molecular Identification of Enterocytozoon bieneusi Isolates from Nigerian Children. J Parasitol Res. 2011; 2011:129542. PMC: 3216387. DOI: 10.1155/2011/129542. View

19.

Sulaiman I, Bern C, Gilman R, Cama V, Kawai V, Vargas D . A molecular biologic study of Enterocytozoon bieneusi in HIV-infected patients in Lima, Peru. J Eukaryot Microbiol. 2004; 50 Suppl:591-6. DOI: 10.1111/j.1550-7408.2003.tb00642.x. View

20.

Jimenez-Gonzalez G, Martinez-Gordillo M, Caballero-Salazar S, Peralta-Abarca G, Cardenas-Cardoz R, Arzate-Barbosa P . [Microsporidia in pediatric patients with leukemia or limphoma]. Rev Invest Clin. 2012; 64(1):25-31. View