Effects of Casein Kinase 2 Alpha 1 Gene Expression on Mice Liver Susceptible to Type 2 Diabetes Mellitus and Obesity

Overview

Journal Int J Med Sci

Specialty General Medicine

Date 2020 Jan 14

PMID 31929734

Citations 7

Authors

Yu-Ching Lan

Yeh-Han Wang

Hsin-Han Chen

Sui-Foon Lo

Shih-Yin Chen

Fuu-Jen Tsai

Affiliations

Soon will be listed here.

Abstract

Diabetes mellitus (DM) is a chronic disease found worldwide. Notably, BKS.Cg- Dock7 Lepr/JNarl mice are useful animal models for studying type 2 diabetes mellitus (T2DM). In this study, we investigated casein kinase 2 alpha 1 (CSNK2A1) gene and protein expression in the liver tissues of mice at different ages (4, 16, and 32 weeks) using real-time quantitative polymerase chain reactions, western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay. Our data paved the way for exploring BKS.Cg- Dock7 +/+ Lepr/JNarl in the mouse model by demonstrating a significant increase in gene and protein expression in T2DM (+Lepr/+Lepr) mouse liver when compared to control (+Dock7/+Dock7) mouse liver. We also observed that CSNK2A1 protein level in the serum of T2DM patient group was higher than that of the control group, although the data was not statistically significant. Based on our findings, we can now understand the role of gene upregulation when encountering T2DM pathologies.

Citing Articles

KD025 Is a Casein Kinase 2 Inhibitor That Protects Against Glucolipotoxicity in β-Cells.

Devkota R, Small J, Carbone K, Glass M, Vetere A, Wagner B Diabetes. 2024; 73(4):585-591.

PMID: 38211571 PMC: 10958584. DOI: 10.2337/db23-0506.

Unraveling the Mechanism of Immunity and Inflammation Related to Molecular Signatures Crosstalk Among Obesity, T2D, and AD: Insights From Bioinformatics Approaches.

Vishal K, Bhuiyan P, Qi J, Chen Y, Zhang J, Yang F Bioinform Biol Insights. 2023; 17:11779322231167977.

PMID: 37124128 PMC: 10134115. DOI: 10.1177/11779322231167977.

Hyperphosphorylation of hepatic proteome characterizes nonalcoholic fatty liver disease in S-adenosylmethionine deficiency.

Robinson A, Binek A, Ramani K, Sundararaman N, Barbier-Torres L, Murray B iScience. 2023; 26(2):105987.

PMID: 36756374 PMC: 9900401. DOI: 10.1016/j.isci.2023.105987.

Protein Kinase CK2 Contributes to Glucose Homeostasis by Targeting Fructose-1,6-Bisphosphatase 1.

Pack M, Gulde T, Volcker M, Boewe A, Wrublewsky S, Ampofo E Int J Mol Sci. 2023; 24(1).

PMID: 36613872 PMC: 9820633. DOI: 10.3390/ijms24010428.

Type 2 Diabetes Mellitus Mediation by the Disruptive Activity of Environmental Toxicants on Sex Hormone Receptors: In Silico Evaluation.

Iheagwam F, Odiba J, Iheagwam O, Ogunlana O, Chinedu S Toxics. 2021; 9(10).

PMID: 34678951 PMC: 8538912. DOI: 10.3390/toxics9100255.

References

Marselli L, Thorne J, Dahiya S, Sgroi D, Sharma A, Bonner-Weir S . Gene expression profiles of Beta-cell enriched tissue obtained by laser capture microdissection from subjects with type 2 diabetes. PLoS One. 2010; 5(7):e11499. PMC: 2903480. DOI: 10.1371/journal.pone.0011499. View

Al-Attar A, Zari T . Influences of crude extract of tea leaves, Camellia sinensis, on streptozotocin diabetic male albino mice. Saudi J Biol Sci. 2013; 17(4):295-301. PMC: 3730800. DOI: 10.1016/j.sjbs.2010.05.007. View

Piazza F, Ruzzene M, Gurrieri C, Montini B, Bonanni L, Chioetto G . Multiple myeloma cell survival relies on high activity of protein kinase CK2. Blood. 2006; 108(5):1698-707. DOI: 10.1182/blood-2005-11-013672. View

Chen H, Charlat O, Tartaglia L, Woolf E, Weng X, Ellis S . Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice. Cell. 1996; 84(3):491-5. DOI: 10.1016/s0092-8674(00)81294-5. View

Lin C, Chen K, Tsai C, Tsai F, Huang C, Tang C . Casticin inhibits human prostate cancer DU 145 cell migration and invasion via Ras/Akt/NF-κB signaling pathways. J Food Biochem. 2019; 43(7):e12902. DOI: 10.1111/jfbc.12902. View

Ruiz de Azua I, Gautam D, Guettier J, Wess J . Novel insights into the function of β-cell M3 muscarinic acetylcholine receptors: therapeutic implications. Trends Endocrinol Metab. 2010; 22(2):74-80. PMC: 3053051. DOI: 10.1016/j.tem.2010.10.004. View

Kim H, Chang Y, Bae H, Eun J, Shen Q, Park S . Oncogenic potential of CK2α and its regulatory role in EGF-induced HDAC2 expression in human liver cancer. FEBS J. 2014; 281(3):851-61. DOI: 10.1111/febs.12652. View

Chen S, Hsu Y, Lin Y, Huang Y, Chen C, Lin W . Current concepts regarding developmental mechanisms in diabetic retinopathy in Taiwan. Biomedicine (Taipei). 2016; 6(2):7. PMC: 4859317. DOI: 10.7603/s40681-016-0007-3. View

Sacco F, Humphrey S, Cox J, Mischnik M, Schulte A, Klabunde T . Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun. 2016; 7:13250. PMC: 5114537. DOI: 10.1038/ncomms13250. View

10.

St-Denis N, Litchfield D . Protein kinase CK2 in health and disease: From birth to death: the role of protein kinase CK2 in the regulation of cell proliferation and survival. Cell Mol Life Sci. 2009; 66(11-12):1817-29. PMC: 11115660. DOI: 10.1007/s00018-009-9150-2. View

11.

Sekiguchi K, Kurabayashi M, Oyama Y, Aihara Y, Tanaka T, Sakamoto H . Homeobox protein Hex induces SMemb/nonmuscle myosin heavy chain-B gene expression through the cAMP-responsive element. Circ Res. 2001; 88(1):52-8. DOI: 10.1161/01.res.88.1.52. View

12.

Steneberg P, Bernardo L, Edfalk S, Lundberg L, Backlund F, Ostenson C . The type 2 diabetes-associated gene ide is required for insulin secretion and suppression of α-synuclein levels in β-cells. Diabetes. 2013; 62(6):2004-14. PMC: 3661649. DOI: 10.2337/db12-1045. View

13.

Hetzel N, Papasouliotis K, Dodkin S, Murphy K . Biochemical assessment of canine body cavity effusions using three bench-top analysers. J Small Anim Pract. 2012; 53(8):459-64. DOI: 10.1111/j.1748-5827.2012.01240.x. View

14.

Chua M, Ortega C, Sheikh A, Lee M, Abdul-Rassoul H, Hartshorn K . CK2 in Cancer: Cellular and Biochemical Mechanisms and Potential Therapeutic Target. Pharmaceuticals (Basel). 2017; 10(1). PMC: 5374422. DOI: 10.3390/ph10010018. View

15.

Jadhao S, Yang R, Lin Q, Hu H, Anania F, Shuldiner A . Murine alanine aminotransferase: cDNA cloning, functional expression, and differential gene regulation in mouse fatty liver. Hepatology. 2004; 39(5):1297-302. DOI: 10.1002/hep.20182. View

16.

Giusiano S, Cochet C, Filhol O, Duchemin-Pelletier E, Secq V, Bonnier P . Protein kinase CK2α subunit over-expression correlates with metastatic risk in breast carcinomas: quantitative immunohistochemistry in tissue microarrays. Eur J Cancer. 2011; 47(5):792-801. DOI: 10.1016/j.ejca.2010.11.028. View

17.

Hummel K, DICKIE M, Coleman D . Diabetes, a new mutation in the mouse. Science. 1966; 153(3740):1127-8. DOI: 10.1126/science.153.3740.1127. View

18.

Tilstra J, Clauson C, Niedernhofer L, Robbins P . NF-κB in Aging and Disease. Aging Dis. 2012; 2(6):449-65. PMC: 3295063. View

19.

Bell L, Chowdhary R, Liu J, Niu X, Zhang J . Integrated bio-entity network: a system for biological knowledge discovery. PLoS One. 2011; 6(6):e21474. PMC: 3124513. DOI: 10.1371/journal.pone.0021474. View

20.

Rossi M, Ruiz de Azua I, Barella L, Sakamoto W, Zhu L, Cui Y . CK2 acts as a potent negative regulator of receptor-mediated insulin release in vitro and in vivo. Proc Natl Acad Sci U S A. 2015; 112(49):E6818-24. PMC: 4679045. DOI: 10.1073/pnas.1519430112. View