In Vivo Analysis of FANCD2 Recruitment at Meiotic DNA Breaks in Caenorhabditis Elegans

Overview

Journal Sci Rep

Specialty Science

Date 2020 Jan 11

PMID 31919410

Citations 8

Authors

Marcello Germoglio

Anna Valenti

Ines Gallo

Chiara Forenza

Pamela Santonicola

Nicola Silva

Adele Adamo

Affiliations

Soon will be listed here.

Abstract

Fanconi Anemia is a rare genetic disease associated with DNA repair defects, congenital abnormalities and infertility. Most of FA pathway is evolutionary conserved, allowing dissection and mechanistic studies in simpler model systems such as Caenorhabditis elegans. In the present study, we employed C. elegans to better understand the role of FA group D2 (FANCD2) protein in vivo, a key player in promoting genome stability. We report that localization of FCD-2/FANCD2 is dynamic during meiotic prophase I and requires its heterodimeric partner FNCI-1/FANCI. Strikingly, we found that FCD-2 recruitment depends on SPO-11-induced double-strand breaks (DSBs) but not RAD-51-mediated strand invasion. Furthermore, exposure to DNA damage-inducing agents boosts FCD-2 recruitment on the chromatin. Finally, analysis of genetic interaction between FCD-2 and BRC-1 (the C. elegans orthologue of mammalian BRCA1) supports a role for these proteins in different DSB repair pathways. Collectively, we showed a direct involvement of FCD-2 at DSBs and speculate on its function in driving meiotic DNA repair.

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