Identification of Plasmodium Falciparum Proteoforms from Liver Stage Models

Overview

Journal Malar J

Publisher Biomed Central

Specialty Tropical Medicine

Date 2020 Jan 9

PMID 31910830

Citations 2

Authors

Benjamin Winer

Kimberly A Edgel

Xiaoyan Zou

Julie Sellau

Sri Hadiwidjojo

Lindsey S Garver

Christin E McDonough

Neil L Kelleher

Paul M Thomas

Eileen Villasante

Alexander Ploss

Vincent R Gerbasi

Affiliations

Soon will be listed here.

Abstract

Background: Immunization with attenuated malaria sporozoites protects humans from experimental malaria challenge by mosquito bite. Protection in humans is strongly correlated with the production of T cells targeting a heterogeneous population of pre-erythrocyte antigen proteoforms, including liver stage antigens. Currently, few T cell epitopes derived from Plasmodium falciparum, the major aetiologic agent of malaria in humans are known.

Methods: In this study both in vitro and in vivo malaria liver stage models were used to sequence host and pathogen proteoforms. Proteoforms from these diverse models were subjected to mild acid elution (of soluble forms), multi-dimensional fractionation, tandem mass spectrometry, and top-down bioinformatics analysis to identify proteoforms in their intact state.

Results: These results identify a group of host and malaria liver stage proteoforms that meet a 5% false discovery rate threshold.

Conclusions: This work provides proof-of-concept for the validity of this mass spectrometry/bioinformatic approach for future studies seeking to reveal malaria liver stage antigens towards vaccine development.

Citing Articles

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In vitro models for human malaria: targeting the liver stage.

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