Role of Donor Genotype in RT-QuIC Seeding Activity of Chronic Wasting Disease Prions Using Human and Bank Vole Substrates

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2020 Jan 8

PMID 31910440

Citations 3

Authors

Soyoun Hwang

Justin J Greenlee

Eric M Nicholson

Affiliations

Soon will be listed here.

Abstract

Chronic wasting disease is a transmissible spongiform encephalopathy of cervids. This fatal neurodegenerative disease is caused by misfolding of the cellular prion protein (PrPC) to pathogenic conformers (PrPSc), and the pathogenic forms accumulate in the brain and other tissues. Real-time Quaking Induced Conversion (RT-QuIC) can be used for the detection of prions and for prion strain discrimination in a variety of biological tissues from humans and animals. In this study, we evaluated how either PrPSc from cervids of different genotypes or PrPSc from different sources of CWD influence the fibril formation of recombinant bank vole (BV) or human prion proteins using RT-QuIC. We found that reaction mixtures seeded with PrPSc from different genotypes of white-tailed deer or reindeer brains have similar conversion efficiency with both substrates. Also, we observed similar results when assays were seeded with different sources of CWD. Thus, we conclude that the genotypes of all sources of CWD used in this study do not influence the level of conversion of PrPC to PrPSc.

Citing Articles

Aqueous extraction of formalin-fixed paraffin-embedded tissue and detection of prion disease using real-time quaking-induced conversion.

Nicholson E, Greenlee J, Hwang S BMC Res Notes. 2024; 17(1):266.

PMID: 39285497 PMC: 11403835. DOI: 10.1186/s13104-024-06886-6.

Standardization of Data Analysis for RT-QuIC-Based Detection of Chronic Wasting Disease.

Rowden G, Picasso-Risso C, Li M, Schwabenlander M, Wolf T, Larsen P Pathogens. 2023; 12(2).

PMID: 36839581 PMC: 9962701. DOI: 10.3390/pathogens12020309.

Hofmeister Effect in RT-QuIC Seeding Activity of Chronic Wasting Disease Prions.

Hwang S, Beckley D, Alekseev K, Nicholson E Front Bioeng Biotechnol. 2021; 9:709965.

PMID: 34660549 PMC: 8515057. DOI: 10.3389/fbioe.2021.709965.

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